Wang Jun, Zhang Cheng-Nan, Xu Xun, Sun Tian-Ci, Kong Ling-Chao, Ning Ren-De
The Third Affiliated Hospital of Anhui Medical University, The First People's Hospital of Hefei, Anhui, Hefei 230000, China.
Hefei University of Technology, Anhui, Hefei 230000, China.
ACS Omega. 2024 Dec 17;9(52):51442-51452. doi: 10.1021/acsomega.4c08537. eCollection 2024 Dec 31.
The challenge of healing diabetic skin wounds presents a significant hurdle in clinical practice and scientific research. In response to this pressing concern, we have developed a temperature-sensitive, in situ-forming hydrogel comprising poly(-isopropylacrylamide---butyl acrylate) -poly(ethylene glycol) -poly(-isopropylacrylamide--butyl acrylate) copolymer, denoted as PEP, in combination with zinc oxide nanoparticles, forming what we refer to as PEP-ZnO hydrogel. The antimicrobial properties of the PEP-ZnO hydrogel against methicillin-resistant were rigorously assessed by using the bacteriostatic banding method. In vitro evaluations encompassed examinations of hemocompatibility and biocompatibility. The study further employed a diabetic Sprague-Dawley (SD) rat whole-layer trauma model for comprehensive in vivo analyses. In vivo healing assessments revealed the potential of the PEP-ZnO hydrogel, characterized by increased collagen deposition and enhanced vascularization at the trauma site, thus significantly expediting the healing process. Collectively, these findings endorse the PEP-ZnO hydrogel as a safe and effective dressing for addressing chronic wounds in diabetic patients. This hydrogel not only holds promise for improving the quality of life for diabetic individuals grappling with chronic wounds but also represents a noteworthy advancement in wound care.
治愈糖尿病皮肤伤口的挑战在临床实践和科学研究中构成了重大障碍。针对这一紧迫问题，我们开发了一种温度敏感型原位形成水凝胶，其由聚（-异丙基丙烯酰胺-丙烯酸丁酯）-聚（乙二醇）-聚（-异丙基丙烯酰胺-丙烯酸丁酯）共聚物（简称PEP）与氧化锌纳米颗粒组成，形成了我们所称的PEP-ZnO水凝胶。通过抑菌圈法严格评估了PEP-ZnO水凝胶对耐甲氧西林菌的抗菌性能。体外评估包括血液相容性和生物相容性检查。该研究进一步采用糖尿病斯普拉格-道利（SD）大鼠全层创伤模型进行全面的体内分析。体内愈合评估揭示了PEP-ZnO水凝胶的潜力，其特点是创伤部位胶原蛋白沉积增加和血管生成增强，从而显著加速愈合过程。总体而言，这些发现认可了PEP-ZnO水凝胶作为治疗糖尿病患者慢性伤口的安全有效敷料。这种水凝胶不仅有望改善患有慢性伤口的糖尿病患者的生活质量，而且代表了伤口护理方面的一项重大进展。
