Persaud Leelawattie, Thenveettil Naflath, Seepaul Ramdeo, Macoon Bisoondat, Reddy Krishna N, Reddy Kambham Raja
Department of Plant and Soil Sciences, 117 Dorman Hall, Box 9555, Mississippi State University, Mississippi State, MS, 39762, USA.
University of Florida, North Florida Research and Education Center, Quincy, FL, 32351, USA.
Heliyon. 2024 Dec 10;10(24):e41113. doi: 10.1016/j.heliyon.2024.e41113. eCollection 2024 Dec 30.
Temperature is a fundamental factor influencing the processes of seed germination. Investigating the response of carinata to thermal stress and establishing a dependable and efficient method for screening thermotolerance will enhance breeding programs and model applications. We assessed the response of 12 carinata genotypes to a range of eight temperatures, spanning from 8 to 37 °C, throughout the germination process. A four-parameter Weibull function effectively characterized the seed germination time course across various temperatures and genotypes. Quadratic functions effectively characterize the maximum seed germination and the rate of seed germination as a function of temperature across various genotypes. The average cardinal temperatures recorded were -0.14, 20.41, and 40.70 °C for maximum seed germination, while the temperatures for seed germination rate were 5.30 °C, 24.51 °C, and 43.71 °C, corresponding to minimum, optimum, and maximum conditions, respectively. A notable variation was observed in maximum seed germination, seed germination rate, and cardinal temperatures across different genotypes. The specified parameters were employed to screen the genotypes for their thermotolerance capabilities. The analysis of principal components and the cumulative response indices effectively characterized carinata genotypes for high- and low-temperature tolerance exhibiting distinct characteristics. The AX17004 was identified as a genotype with significant tolerance to high temperatures, whereas AX17009 demonstrated remarkable tolerance to low temperatures. In contrast, AX17002 exhibited sensitivity to both high and low-temperature stress. According to the classification of breed types, both double haploid and hybrid groups revealed consistent thermotolerance responses during the germination phase. In contrast, the inbred group exhibited a wider response cluster to both minimum and maximum temperatures. The assay method presents a cost-effective approach for evaluating thermotolerance in carinata genotypes.
温度是影响种子萌发过程的一个基本因素。研究油菜对热胁迫的响应并建立一种可靠且高效的耐热性筛选方法，将有助于改进育种计划和模型应用。我们评估了12个油菜基因型在8至37°C的8种温度范围内整个萌发过程中的响应。一个四参数威布尔函数有效地描述了不同温度和基因型下的种子萌发时间进程。二次函数有效地描述了不同基因型下最大种子萌发率和种子萌发速率随温度的变化。记录的最大种子萌发的平均基点温度分别为-0.14、20.41和40.70°C，而种子萌发速率的温度分别为5.30°C、24.51°C和43.71°C，分别对应最低、最适和最高条件。不同基因型在最大种子萌发率、种子萌发速率和基点温度方面存在显著差异。利用指定参数筛选基因型的耐热能力。主成分分析和累积响应指数有效地描述了油菜基因型在高温和低温耐受性方面的不同特征。AX17004被鉴定为对高温具有显著耐受性的基因型，而AX17009对低温表现出显著耐受性。相比之下，AX17002对高温和低温胁迫均敏感。根据品种类型分类，双单倍体和杂交组在萌发阶段均表现出一致的耐热性响应。相比之下，自交组对最低和最高温度均表现出更广泛的响应聚类。该测定方法为评估油菜基因型的耐热性提供了一种经济有效的方法。
