van Kessel K, Castelijn G, van der Voort M, Meijer N
Wageningen Food Safety Research, Akkermaalsbos 2, P.O. box 230, 3700 AE, Wageningen, the Netherlands.
Heliyon. 2024 Dec 7;10(24):e40912. doi: 10.1016/j.heliyon.2024.e40912. eCollection 2024 Dec 30.
Insects are increasingly used as an alternative protein source for feed and food production. One of the main biological hazards associated with edible insects is the bio-accumulation of foodborne pathogenic microorganisms. In this study, the interaction of larvae of the black soldier fly (BSFL, (L.), Diptera: Stratiomyidae) with the foodborne pathogen was explored. As such, BSFL were reared on a substrate of wheat-based insect feed mixed with water, which was inoculated with either vegetative cells or endospores After seven days of rearing, the larvae and the residual substrate (frass) were analyzed for the presence of , phenotypically via classical microbial counting and genotypically via real-time PCR. Endospores were detected on a selective growth medium in the larvae as well as in the frass. An additional heating step (1 min at 100 °C) to mimic blanching did not reduce the microbial count of the endospores. Results show that endospores can be transferred to larvae. It is therefore recommend that substrate ingredients for BSFL rearing are tested for the presence of endospores.
昆虫越来越多地被用作饲料和食品生产的替代蛋白质来源。与可食用昆虫相关的主要生物危害之一是食源性病原体的生物累积。在本研究中，探索了黑水虻幼虫（双翅目：水虻科）与食源性病原体的相互作用。因此，将黑水虻幼虫饲养在混合了水的小麦基昆虫饲料底物上，该底物接种了营养细胞或芽孢。饲养七天后，通过经典微生物计数从表型上以及通过实时PCR从基因型上分析幼虫和残留底物（粪便）中是否存在该病原体。在幼虫以及粪便中的选择性生长培养基上检测到了芽孢。模拟热烫的额外加热步骤（100℃ 1分钟）并未降低芽孢的微生物数量。结果表明，该芽孢可转移至幼虫。因此，建议对黑水虻饲养的底物成分进行该芽孢存在情况的检测。
