Lopes Ivã Guidini, Lalander Cecilia, Vidotti Rose Meire, Vinnerås Björn
Aquaculture Center of Unesp (Caunesp), São Paulo State University (UNESP), São Paulo, Brazil.
Department of Energy and Technology, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Front Microbiol. 2020 Jul 16;11:1616. doi: 10.3389/fmicb.2020.01616. eCollection 2020.
This study evaluated the impact of feeding regimes on process performance and inactivation of microorganisms during treatment of aquaculture waste with black soldier fly (BSF) larvae. In three treatments (T1-T3), a blend of reclaimed bread and aquaculture waste was used as substrate for BSF larvae. In T1, the substrate was inoculated with four subtypes of spp. and (both at 1% /), and offered only once, at the beginning of the 14-day trial. In T2 and T3, the substrate was supplied on three different days, with contaminated substrate provided only the first event in T2 and in all three events in T3. Provision of a lump sum feeding (T1) proved unfavorable for larval growth and process efficiency, but did not affect the microbial reduction effect. The total reduction in spp. was approximately 6 log in T1 and T2, and 3.3 log in T3, while the total reduction in was approximately 4 log in T1 and T2, and 1.9 log in T3. After removing the larvae, the treatment residues were re-inoculated with spp. and . It was found that the inactivation in both organisms continued in all treatments that originally contained BSF larvae (T1-T3), suggesting that antimicrobial substances may have been secreted by BSF larvae or by its associated microbiota.
本研究评估了投喂方式对黑水虻（BSF）幼虫处理水产养殖废弃物过程中工艺性能和微生物灭活的影响。在三种处理方式（T1 - T3）中，将回收面包和水产养殖废弃物的混合物用作BSF幼虫的底物。在T1中，底物接种了四种亚型的 spp. 和（均为1% /），且仅在14天试验开始时投喂一次。在T2和T3中，底物在三个不同日期供应，受污染的底物仅在T2的第一次投喂时提供，在T3的所有三次投喂时提供。一次性投喂（T1）对幼虫生长和工艺效率不利，但不影响微生物减少效果。T1和T2中 spp. 的总减少量约为6个对数，T3中为3.3个对数；而T1和T2中 的总减少量约为4个对数，T3中为1.9个对数。去除幼虫后，处理后的残渣重新接种了 spp. 和 。结果发现，在所有最初含有BSF幼虫的处理方式（T1 - T3）中，两种微生物的灭活仍在继续，这表明黑水虻幼虫或其相关微生物群可能分泌了抗菌物质。
