布基纳法索瓦加杜古市三个生物群落中致病性肠杆菌菌株的流行情况及抗生素敏感性
Prevalence and Antibiotic Susceptibility of Pathogenic Enterobacteria Strains from Three Biotopes in the City of Ouagadougou (Burkina Faso).
作者信息
Dabiré Sanhitouo Charlemagne, Somda Marius K, Nitièma Léon W, Kambiré Dinanibè, Kiemtoré Samiratou, Soubeiga Serge Théophile, Zouré Abdou Azaque, Yao Konan Kouakou Toussaint, Compaoré Tegwindé Rebeca, Ouedraogo Henri Gautier, Dicko Mamoudou H
机构信息
Département Biomédical et Santé publique, Institut de Recherche en Sciences de la Santé ´(IRSS)/Centre National de la Recherche Scientifique et Technologique (CNRST), Ouagadougou, Burkina Faso.
Département de Biochimie Microbiologie, Ecole Doctorale Sciences Et Technologies (EDST)/Université Joseph KI-ZERBO, Ouagadougou, Burkina Faso.
出版信息
Infect Drug Resist. 2024 Dec 30;17:5909-5924. doi: 10.2147/IDR.S485200. eCollection 2024.
PURPOSE
The emergence of antibiotic resistance in pathogenic is a public health problem in tropical countries such as Burkina Faso. Antibiotic resistance could be identified using a variety of approaches. This study aimed to estimate the prevalence of pathogenic enterobacteria strains from three sources, as well as their antibiotic resistance profile to biotope and climatic season.
MATERIAL AND METHODS
The methodological approach consisted of identifying from human (urine, stool), animal (eggs, milk, fish), and environmental (soil, lettuce) samples, followed by assessing their antibiotic susceptibility. Samples were collected from February to December 2023. Bacterial species were isolated and phenotypically identified (morphologically, culturally, biochemically, and antigenically) using standard methods. The prevalence of bacterial susceptibility to ten antibiotics was determined using the agar disk diffusion method. The collected data were analyzed with IBM SPSS Statistics 25 software.
RESULTS
A total of 615 isolates were collected, including 300, 168, and 147 samples from human, animal, and environmental sources respectively. Phenotypic characteristics allowed to partially identify 43 species, among these 29.76% belonged to , 24.72% , 13. and 2.6% to . Bacterial resistance rates were: aminopenicillins (54.8%), first-generation cephalosporins (35.3%), sulfonamides (33.3%), third-generation cephalosporins (30.7%), fourth-generation cephalosporins (22.5%), fluoroquinolones (21.8%), phenicols (16.8%), and carbapenems (16.2%). The distribution of antibiotic resistance was 45.3% from human sources, 19.3% from animal sources, and 13.8% from environmental sources.
CONCLUSION
The results indicate that resistant bacteria can come from any of the three biotopes, with human origin being the most frequent. The high prevalence of resistance to the antibiotics tested in isolated bacteria raises interest in investigating the genetic factors responsible.
目的
在布基纳法索等热带国家，病原菌中抗生素耐药性的出现是一个公共卫生问题。抗生素耐药性可通过多种方法来识别。本研究旨在估计来自三种来源的致病性肠杆菌菌株的流行率，以及它们对生物群落和气候季节的抗生素耐药谱。
材料与方法
该方法包括从人类（尿液、粪便）、动物（鸡蛋、牛奶、鱼类）和环境（土壤、生菜）样本中识别细菌，随后评估它们的抗生素敏感性。样本于2023年2月至12月采集。使用标准方法分离细菌物种并进行表型鉴定（形态学、培养、生化和抗原学方面）。采用琼脂纸片扩散法测定细菌对十种抗生素的敏感性流行率。收集的数据使用IBM SPSS Statistics 25软件进行分析。
结果
共收集到615株细菌分离株，分别来自人类、动物和环境来源的样本有300份、168份和147份。表型特征使我们能够部分鉴定出43个物种，其中29.76%属于大肠杆菌，24.72%属于肺炎克雷伯菌，13.17%属于奇异变形杆菌，2.6%属于阴沟肠杆菌。细菌耐药率分别为：氨基青霉素（54.8%）、第一代头孢菌素（35.3%）、磺胺类（33.3%）、第三代头孢菌素（30.7%）、第四代头孢菌素（22.5%）、氟喹诺酮类（21.8%）、酚类（16.8%）和碳青霉烯类（16.2%）。抗生素耐药性的分布情况为：人类来源占45.3%，动物来源占19.3%，环境来源占13.8%。
结论
结果表明，耐药菌可来自这三种生物群落中的任何一种，其中人类来源最为常见。分离出的细菌对所测试抗生素的高耐药率引发了对研究其相关遗传因素的兴趣。
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