Kamchoo Kanda, Hualkasin Watcharasuda, Pathaburee Pojchanad, Pachanawan Adithepchaikarn
Faculty of Innovative Agriculture, Fisheries and Food, Prince of Songkla University, Surat Thani Campus, Surat Thani, Thailand.
Faculty of Science and Industrial Technology, Prince of Songkla University, Surat Thani Campus, Surat Thani, Thailand.
Folia Parasitol (Praha). 2024 Dec 5;71:2024.024. doi: 10.14411/fp.2024.024.
The nematode Spinitectus notopteri Karve et Naik, 1951 was collected from two species of freshwater fish belonging to the family Notopteridae, namely Chitala ornata (Gray) and Notopterus notopterus (Pallas), at Khun Thale Swamp in Surat Thani province of southern Thailand. The overall prevalence of the parasite was found to be 88% (94 fish infected/106 fish examined). A higher prevalence was found in C. ornata (96%), while a higher intensity was noted for N. notopterus (117 parasites/fish). The most important morphological characters were the presence of cephalic and cuticular structures, precloacal ridges, and genital papillae. Specimens were genetically characterised using cox1 mtDNA. Morphological characteristics were most similar to Spinitectus petterae Boomker, 1993 from Clarias gariepinus (Burchell) in Africa, while the genetic data were dissimilar to all available data for the genus. Therefore, the identification of nematode specimens using a combination of morphological and molecular techniques is stressed. This study also presents the first molecular analysis of S. notopteri infecting Thai freshwater fish and a new geographical record for S. notopteri.
线虫斯皮尼线虫（Spinitectus notopteri Karve et Naik，1951）于泰国南部素叻他尼府的昆他莱沼泽，从两种属于弓背鱼科的淡水鱼中采集到，即美丽骨舌鱼（Chitala ornata (Gray)）和弓背鱼（Notopterus notopterus (Pallas)）。发现该寄生虫的总体感染率为88%（106条检查鱼中有94条感染）。在美丽骨舌鱼中发现了更高的感染率（96%），而弓背鱼的感染强度更高（每条鱼有117个寄生虫）。最重要的形态特征是存在头部和表皮结构、泄殖腔前嵴和生殖乳头。使用细胞色素氧化酶亚基1线粒体DNA（cox1 mtDNA）对标本进行了基因特征分析。形态特征与来自非洲的尖吻鲈（Clarias gariepinus (Burchell)）的1993年的彼得斯斯皮尼线虫（Spinitectus petterae）最为相似，而基因数据与该属所有现有数据不同。因此，强调结合形态学和分子技术对线虫标本进行鉴定。本研究还首次对感染泰国淡水鱼的斯皮尼线虫进行了分子分析，并提供了斯皮尼线虫的新地理记录。
