El-Mansy Salah A, Naiel Mohammed A E, Abu El-Naser Ibrahim A, De Waard Michel, Babalghith Ahmad O, Ogaly Hanan A, El-Saber Batiha Gaber, Ghazy Abdullah A
Department of Animal Production, Faculty of Agriculture, Zagazig University, Zagazig, 44519, Egypt.
Animal Production Department, Faculty of Agriculture, Damietta University, Damietta, Egypt.
Heliyon. 2023 Mar 2;9(3):e14194. doi: 10.1016/j.heliyon.2023.e14194. eCollection 2023 Mar.
The main purpose of the existing experiment was to assess the allelic and genotypic polymorphisms of the Growth hormone (GH) gene and its correlation with growth indices, efficiency of consumed feed, some body indices and carcass traits of the Egyptian Awassi sheep. Forty Egyptian Awassi male lambs were selected for their growing indices (post-weaning daily gain, marketing weight, and weaning weight), feed efficiency (consumed feed and of consumed feed), body conformational indicators (skeletal muscle index, relative body index, body mass index, and body index), and carcass features (dressing %, hot carcass weight, fore-legs %, neck %, loin %, ribs %, abdominal fat %, Tail % and hind-legs %). The polymerase chain reaction-restricted fragment length polymorphism (PCR-RFLP) tool was applied to detect the genotypic and allelic GH gene polymorphisms properties. The PCR-RFLP analysis identified three main genotypes (AA, AB and BB) and two main alleles (A and B). The GH genotype (AA or AB) exhibited moderate significant influence (P < 0.05) on marketing weight, Loin% and Tail%. Also, genotype (AB or BB) of expressed GH gene significantly (P < 0.01) influence on consumed feed, post-weaning daily gain, neck% and body mass index, whereas, it had no influence on the phenotypic values of the other characteristics investigated. The presence of the A allele in the genotype was markedly associated (P < 0.01) with consumed feed, body mass index and post-weaning daily growth; conversely, the presence of the B allele in the genotype was significantly related (P < 0.05) with marketing weight, loin %, and tail %. While, GH gene expression was revealed to be highly significant (P < 0.01) in relation to post-weaning daily growth, feed intake, and neck %. According to the findings, determining the associations between GH gene variation and growth, efficiency of consumed feed, body measurements, and carcass features of Egyptian Awassi sheep and applying marker assisted selection with the GH gene to improve these traits is warranted and will be of significant economic value to sheep production.
现有实验的主要目的是评估生长激素（GH）基因的等位基因和基因型多态性，及其与埃及阿瓦西羊的生长指标、饲料消耗效率、一些身体指标和胴体性状的相关性。根据生长指标（断奶后日增重、上市体重和断奶体重）、饲料效率（消耗饲料量和饲料转化率）、身体形态指标（骨骼肌指数、相对身体指数、体重指数和体指数）以及胴体特征（屠宰率、热胴体重、前腿比例、颈部比例、腰部比例、肋骨比例、腹部脂肪比例、尾部比例和后腿比例），挑选了40只埃及阿瓦西雄性羔羊。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术检测GH基因的基因型和等位基因多态性特征。PCR-RFLP分析确定了三种主要基因型（AA、AB和BB）和两个主要等位基因（A和B）。GH基因型（AA或AB）对上市体重、腰部比例和尾部比例有中度显著影响（P < 0.05）。此外，表达GH基因的基因型（AB或BB）对消耗饲料量、断奶后日增重、颈部比例和体重指数有显著影响（P < 0.01），而对其他所研究的表型值没有影响。基因型中A等位基因的存在与消耗饲料量、体重指数和断奶后日生长显著相关（P < 0.01）；相反，基因型中B等位基因的存在与上市体重、腰部比例和尾部比例显著相关（P < 0.05）。同时，GH基因表达与断奶后日生长、采食量和颈部比例高度显著相关（P < 0.01）。根据研究结果，确定GH基因变异与埃及阿瓦西羊的生长、饲料消耗效率、身体测量和胴体特征之间的关联，并应用GH基因进行标记辅助选择以改善这些性状是有必要的，这将对绵羊生产具有重要的经济价值。
