Geoshi Basma El, El-Akabawy Gehan, Metwally Mohammed El, Soliman Magda I
Mansoura Medical Research Center (MERC), Faculty of Medicine, Mansoura University, Mansoura, Egypt.
Department of Basic Medical Sciences, College of Medicine, Centre of Medical and Bioallied Health Sciences Research, Ajman University, Ajman, United Arab Emirates.
BMC Genomics. 2025 Jan 6;26(1):9. doi: 10.1186/s12864-024-11154-9.
The current study aimed to detect the mutagenic impacts of aflatoxin B1 (AFB1), which is produced by Aspergillus group fungi, via a high-plant genotoxicity test. Different durations of treatment (3 h, 6 h, and 12 h) were used to treat the Vicia faba root tips with varying concentrations of Aflatoxin B1 (AFB1) following the approved protocol for plant assays published by the International Program on Chemical Safety (IPCS) and the World Health Organization (WHO). The data obtained indicated that AFB1 not only has the ability to induce various alterations in the process of mitosis, ranging from increasing to decreasing mitotic and phase indices but also leads to many mitotic aberrations. The abnormalities observed varied on the basis of the ratio of AFB1 to treatment time. The aberrations included micronuclei in interphase, stickiness; two groups ring star disturbed and oblique metaphase late separation diagonal bridge and laggard and disturbed. anaphase and telophase. This study showed that biomonitoring Vicia faba is a sustainable method for estimating the cytotoxicity and genotoxicity of applied AFB1. Additionally, AFB1 caused changes in the protein profile detected by SDS‒PAGE, with each treated sample displaying a unique electrophoretic pattern due to the formation and disappearance of certain bands. The ISSR and RAPD assays changes in band numbers in all samples compared with the untreated control, and a decrease in genetic template stability (GTS) ratios was observed with higher levels of AFB1. The image cytometric data revealed a correlation between the dosage of AFB1 and its impact on cell cycle components in the meristematic cells of Vicia faba roots. Furthermore, an increase in AFB1 concentrationled to a decrease in B-cell lymphoma 2 (Bcl2) levels, an increase in chromatin condensation levels, and an increase in poly ADP‒ribose polymorphism (PARP) levels.
本研究旨在通过一项高等植物遗传毒性试验，检测由曲霉菌群真菌产生的黄曲霉毒素B1（AFB1）的致突变影响。按照国际化学品安全规划署（IPCS）和世界卫生组织（WHO）发布的植物检测批准方案，使用不同浓度的黄曲霉毒素B1（AFB1）处理蚕豆根尖，处理时间分别为3小时、6小时和12小时。获得的数据表明，AFB1不仅能够在有丝分裂过程中诱导各种变化，从增加到减少有丝分裂和分期指数，还会导致许多有丝分裂畸变。观察到的异常情况因AFB1与处理时间的比例而异。这些畸变包括间期微核、粘连；两组环形星状干扰和斜中期后期分离对角桥以及落后和干扰的后期和末期。本研究表明，蚕豆生物监测是评估所应用AFB1的细胞毒性和遗传毒性的一种可持续方法。此外，AFB1导致通过SDS-PAGE检测的蛋白质谱发生变化，由于某些条带的形成和消失，每个处理过的样品都呈现出独特的电泳模式。与未处理的对照相比，所有样品的ISSR和RAPD分析条带数量发生变化，并且随着AFB1水平的升高，观察到遗传模板稳定性（GTS）比率降低。图像细胞计量学数据揭示了AFB1剂量与其对蚕豆根分生细胞中细胞周期成分的影响之间的相关性。此外，AFB1浓度的增加导致B细胞淋巴瘤2（Bcl2）水平降低、染色质凝聚水平增加以及聚ADP-核糖多态性（PARP）水平增加。
