Sziklai I, Gróf J, Ribári O, Menyhárt J, Piffko P
Acta Otolaryngol. 1985 Jan-Feb;99(1-2):25-34. doi: 10.3109/00016488509119142.
Perilymphs of normal and otosclerotic origin were separated chromatographically on a Sephadex G-25 microcolumn. Peptide composition of the perilymphs was compared by capillary analytical isotachophoresis in the molecular mass range 0.3-5 kD. Otosclerotic perilymph samples contain a heterogeneous, UV-absorbing peptide subfraction which is not detected in the normal perilymph. Normal and otosclerotic perilymph, furthermore, contain four common subfractions detected in twice the normal concentration in the otosclerotic perilymph. These ITP subfractions are degraded during acid hydrolysis (6 M HCI). On the contrary, otosclerosis is a deficient state compared with the normal, as the number of peptides or oligoglycopeptides is twice as high in normal as in otosclerotic perilymph, beside the otosclerosis specific peptides.
将正常来源和耳硬化症来源的外淋巴在Sephadex G - 25微柱上进行色谱分离。通过毛细管分析等速电泳在0.3 - 5 kD分子量范围内比较外淋巴的肽组成。耳硬化症外淋巴样本含有一种异质性的、吸收紫外线的肽亚组分，而在正常外淋巴中未检测到。此外，正常和耳硬化症外淋巴含有四个共同的亚组分，在耳硬化症外淋巴中的浓度是正常浓度的两倍。这些等速电泳亚组分在酸水解（6 M HCl）过程中会降解。相反，与正常情况相比，耳硬化症是一种缺乏状态，因为除了耳硬化症特异性肽之外，正常外淋巴中的肽或寡糖肽数量是耳硬化症外淋巴中的两倍。
