Gróf J, Sziklai I, Ribári O, Menyhárt J
Acta Otolaryngol. 1985 May-Jun;99(5-6):557-63. doi: 10.3109/00016488509182261.
Non-collagen proteins extracted from the otosclerotic stapes footplate, superstructure and temporal cortical bone were compared with the protein patterns of normal and otosclerotic perilymph by analytical isotachophoresis (ITP). Normal and otosclerotic perilymph yielded basically identical isotachophoretic subfractions. Thirteen subfractions were detected moving as anions at pH 9.6 in the normal perilymph, versus 16 subfractions of similar character in the otosclerotic perilymph. Of these 16 protein subfractions, one (which was not detected in the normal perilymph) could be found in the ITP-gram of the stapes footplate non-collagen proteins, but not of the stapes superstructure or temporal cortical bone. Our results support the concept that protein can enter the inner ear fluid spaces from the otosclerotic bone. The biological significance of these proteins has not yet been clarified.
通过分析等速电泳（ITP），对从耳硬化镫骨足板、上部结构和颞骨皮质骨中提取的非胶原蛋白与正常和耳硬化外淋巴的蛋白质模式进行了比较。正常和耳硬化外淋巴产生的等速电泳亚组分基本相同。在pH 9.6条件下，正常外淋巴中有13个亚组分作为阴离子移动，而耳硬化外淋巴中有16个类似性质的亚组分。在这16个蛋白质亚组分中，有一个（在正常外淋巴中未检测到）可在镫骨足板非胶原蛋白的ITP图谱中发现，但在镫骨上部结构或颞骨皮质骨的ITP图谱中未发现。我们的结果支持蛋白质可从耳硬化骨进入内耳液间隙的概念。这些蛋白质的生物学意义尚未阐明。
