Ribári O, Sziklai I
Department of Otorhinolaryngology, Semmelweis University Medical School, Budapest, Hungary.
Acta Otolaryngol. 1988 May-Jun;105(5-6):549-52. doi: 10.3109/00016488809119518.
Otosclerotic bone and perilymph samples obtained during stapedectomy operations from patients suffering from pure conductive hearing loss of at least 40 dBs air-bone gap at 500 Hz. Control perilymph samples derived from patients suffering from round window membrane rupture or Meniere's disease. Enzyme activities were determined using a synthetic fluorogenic substrate by fluorescence spectrophotometry. No significant differences have been found between the protein content of otosclerotic and normal or non-otosclerotic perilymph samples. Cathepsin D activity was significantly higher (p less than 0.001) in the otosclerotic perilymph samples. Otosclerotic stapes footplate contained more activity than normal meatal cortical bone as well, though the difference was not significant. Biological significance of the elevated Cathepsin D activity must be considered.
从患有至少40分贝气骨导差（500赫兹时）的纯传导性听力损失患者的镫骨切除术手术中获取耳硬化骨和外淋巴样本。对照外淋巴样本取自患有圆窗膜破裂或梅尼埃病的患者。使用合成荧光底物通过荧光分光光度法测定酶活性。耳硬化外淋巴样本与正常或非耳硬化外淋巴样本的蛋白质含量之间未发现显著差异。耳硬化外淋巴样本中的组织蛋白酶D活性显著更高（p小于0.001）。耳硬化镫骨足板的活性也高于正常耳道皮质骨，尽管差异不显著。必须考虑组织蛋白酶D活性升高的生物学意义。
