Elssaig Elmutuz H, Ahmed-Abakur Eltayib H, Alnour Tarig M S, Alsubai Mohamed A, Yousif Aadil
Department of Medical Laboratory Technology, University of Tabuk, Tabuk, Saudi Arabia.
Molecular Microbiology Lab, Prince Fahad Bin Sultan Chair for Biomedical Research, University of Tabuk, Tabuk, Saudi Arabia.
J Res Med Sci. 2024 Nov 28;29:71. doi: 10.4103/jrms.jrms_717_23. eCollection 2024.
The study aimed to detect the association between insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) and interleukin-6 (IL-6) polymorphisms among type 2 diabetes mellitus (T2DM).
This study involved 500 individuals; 250 obese DM cases and 250 healthy controls. The polymerase chain reaction restriction fragment length polymorphism was used to identify the genotype of the IGF2BP2 gene for the small nucleoproteins rs4402960 (G>T) and small nucleoproteins rs800795 (G>C).
The results indicated that the mutant C-allele of the single nucleotide polymorphism (SNP) rs1800795 variant was highly significantly associated with the study group ( = 0.002). The occurrence of genotypes (C/G and C/C) versus normal G/G genotype of the IL-6 variant was highly significant ( = 0.004) in the study group (49.2% vs. 50.8%) compared to the control group (62% vs. 38%). Similar findings were observed in SNPs for the rs440960 variant, in which the T-allele indicated a highly significant relationship ( = 0.0001) with the study group; the frequency of G/G genotype versus both (T/T and G/T) genotypes was highly significant ( = 0.0001) in the case group (34% vs. 66%) than in the healthy control group (52% vs. 48%).
The current study indicated that IGF2BP2 rs4402960 and IL-6 rs1800795 polymorphism were highly significantly associated with the increased risk of obese T2DM among the Saudi Arabian population and presented a genetic model to screen the high-risk individuals with further validations.
本研究旨在检测2型糖尿病（T2DM）患者中胰岛素样生长因子2 mRNA结合蛋白2（IGF2BP2）与白细胞介素-6（IL-6）基因多态性之间的关联。
本研究纳入500名个体，其中250例肥胖糖尿病患者和250名健康对照者。采用聚合酶链反应-限制性片段长度多态性方法鉴定小核核糖核蛋白rs4402960（G>T）和小核核糖核蛋白rs800795（G>C）的IGF2BP2基因的基因型。
结果表明，单核苷酸多态性（SNP）rs1800795变体的突变C等位基因与研究组高度显著相关（P = 0.002）。与对照组（62%对38%）相比，研究组中IL-6变体的基因型（C/G和C/C）与正常G/G基因型的发生情况高度显著（P = 0.004）（49.2%对50.8%）。在rs440960变体的SNP中也观察到类似结果，其中T等位基因与研究组有高度显著关系（P = 0.0001）；病例组中G/G基因型与（T/T和G/T）基因型的频率相比健康对照组（52%对48%）有高度显著差异（P = 0.0001）（34%对66%）。
当前研究表明，IGF2BP2 rs4402960和IL-6 rs1800795多态性与沙特阿拉伯人群中肥胖TIDM风险增加高度显著相关，并提出了一个遗传模型，有待进一步验证以筛选高危个体。
