Shi Yujie, Chen Zhen, Jiang Jingyong, Wu Wenwu, Xin Yue, Zeng Wei
Zhejiang Provincial Key Laboratory of Plant Evolutionary Ecology and Conservation, College of Life Sciences, Taizhou University, Taizhou, China.
Institute of Horticulture, Taizhou Academy of Agricultural Sciences, Linhai, China.
Front Plant Sci. 2024 Dec 23;15:1504687. doi: 10.3389/fpls.2024.1504687. eCollection 2024.
var. is a special berry plant of in the Rosaceae family. Its leaves contain high-sweetness, low-calorie, and non-toxic sweet ingredients, known as rubusoside. As a medicine and food biofunctional plant, it is a combination of "tea, sugar, and medicine." In this study, the complete mitogenome of var. was successfully assembled and annotated based on PacBio HiFi sequencing technology. The mitogenome of var. was a typical master circle structure, spanning 432,483 bp and containing 34 unique protein-coding genes (PCGs), 20 tRNAs, and 3 rRNAs. The majority of these PCGs was subjected to purifying selection, and only one gene () showed sign of positive selection. The mitogenome of var. contained a large number of repeats, and the homogeneous fragments transferring between plastid genome and mitogenome, with a total of 55 pairs of mitochondrial plastid sequences (MTPTs), and the total size was 56,913 bp. Comparative analysis showed that the non-coding region in the mitogenome of var. had undergone frequent rearrangements during evolution, but the coding region was still highly conserved. Furthermore, the maximum likelihood and Bayesian inference phylogenetic trees were reconstructed of 10 shared PCGs in 36 plant species. The topological structures of two phylogenetic trees were consistent with the APG IV classification system and had high support rates. In general, this study clarifies the mitogenome of var. and provides valuable insights into the genetic evolution of the Rosaceae family.
变种是蔷薇科的一种特殊浆果植物。其叶子含有高甜度、低热量且无毒的甜味成分，即悬钩子苷。作为一种药食两用的生物功能植物，它是“茶、糖、药”的结合体。在本研究中，基于PacBio HiFi测序技术成功组装并注释了变种的完整线粒体基因组。变种的线粒体基因组是典型的主环结构，跨度为432,483 bp，包含34个独特的蛋白质编码基因（PCG）、20个tRNA和3个rRNA。这些PCG中的大多数受到纯化选择，只有一个基因（）显示出正选择的迹象。变种的线粒体基因组包含大量重复序列，质体基因组和线粒体基因组之间存在同源片段转移，共有55对线粒体质体序列（MTPT），总大小为56,913 bp。比较分析表明，变种线粒体基因组中的非编码区在进化过程中经历了频繁的重排，但编码区仍然高度保守。此外，基于36种植物中10个共享PCG重建了最大似然法和贝叶斯推断系统发育树。两棵系统发育树的拓扑结构与APG IV分类系统一致，支持率较高。总体而言，本研究阐明了变种的线粒体基因组，为蔷薇科的遗传进化提供了有价值的见解。
