• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

枳椇子汤通过调节肠道微生物群及其相关代谢产物，并抑制TLR4/NF-κB/NLRP3通路来改善溃疡性结肠炎。

Zhili decoction ameliorates ulcerative colitis by modulating gut microbiota and related metabolite, and inhibiting the TLR4/NF-κB/NLRP3 pathway.

作者信息

Tan Tianying, Chen Qin, Chen Ping, Li Shuangshuang, Hu Wenting, Yang Tao, Jia Yingtian

机构信息

College of Clinical Medicine, Guizhou University of Traditional Chinese Medicine, Guiyang, China.

Department of Anorectal, Kunming Municipal Hospital of Traditional Chinese Medicine, The Third Affiliated Hospital of Yunnan University of Chinese Medicine, Kunming, China.

出版信息

Front Pharmacol. 2024 Dec 23;15:1481273. doi: 10.3389/fphar.2024.1481273. eCollection 2024.

DOI:10.3389/fphar.2024.1481273
PMID:39764470
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11701441/
Abstract

ETHNOPHARMACOLOGICAL IMPORTANCE

Zhili decoction (ZLD) is a traditional Chinese medicine prescription for ulcerative colitis (UC). However, the mechanism by which ZLD exerts its therapeutic effects in the context of UC remains unclear.

AIM OF STUDY

The aim of this study was to investigate the effects of ZLD on the gut microbiota and related fecal metabolite levels using a mouse model of UC. In addition, we examined the underlying molecular mechanisms responsible for these effects.

MATERIALS AND METHODS

The major components of ZLD were detected by ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). An integrated approach employing 16S rRNA and fecal metabolomics was employed to assess the potential impacts of ZLD on gut flora abundance and diversity, fecal metabolite levels, and various metabolic pathways. To further investigate the potential mechanisms of ZLD in treating UC, the expression of genes and proteins were examined by RT-qPCR, immunohistochemical staining and Western blotting.

RESULTS

ZLD markedly alleviated symptoms and inflammatory injury in mice with UC. DSS induced notable alterations in the gut microbiome, and ZLD enhanced gut microbial diversity in UC mice by augmenting the abundance of Bacteroidota, Christensenella, , etc., while reducing the / ratio. ZLD treatment significantly modified the metabolic profile of mice with UC. It significantly suppressed the arachidonic acid metabolic pathway and promoted the butyrate acid metabolic pathway. ZLD reduced inflammatory factors and inhibited TLR4/NF-κB/NLRP3 pathway expression. In addition, correlation analysis demonstrated a close relationship between gut microbes, fecal metabolites, and inflammatory factors.

CONCLUSION

ZLD alleviated UC by regulating gut flora, modulating related metabolite levels, and inhibiting TLR4/NF-κB/NLRP3 pathway.

摘要

民族药理学重要性

枳椇子汤（ZLD）是一种用于治疗溃疡性结肠炎（UC）的中药方剂。然而，ZLD在UC背景下发挥治疗作用的机制尚不清楚。

研究目的

本研究旨在使用UC小鼠模型研究ZLD对肠道微生物群及相关粪便代谢物水平的影响。此外，我们还研究了造成这些影响的潜在分子机制。

材料与方法

采用超高效液相色谱-质谱联用仪（UPLC-MS）检测ZLD的主要成分。采用16S rRNA和粪便代谢组学的综合方法评估ZLD对肠道菌群丰度和多样性、粪便代谢物水平以及各种代谢途径的潜在影响。为了进一步研究ZLD治疗UC的潜在机制，通过RT-qPCR、免疫组织化学染色和蛋白质印迹法检测基因和蛋白质的表达。

结果

ZLD显著减轻了UC小鼠的症状和炎症损伤。葡聚糖硫酸钠（DSS）诱导肠道微生物群发生显著变化，ZLD通过增加拟杆菌门、克里斯滕森菌属等的丰度，同时降低/比值，增强了UC小鼠的肠道微生物多样性。ZLD治疗显著改变了UC小鼠的代谢谱。它显著抑制花生四烯酸代谢途径，促进丁酸代谢途径。ZLD降低了炎症因子水平，抑制了TLR4/NF-κB/NLRP3信号通路的表达。此外，相关性分析表明肠道微生物、粪便代谢物和炎症因子之间存在密切关系。

结论

ZLD通过调节肠道菌群、调节相关代谢物水平和抑制TLR4/NF-κB/NLRP3信号通路来缓解UC。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9026/11701441/ecd14aa0d3df/fphar-15-1481273-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9026/11701441/ce72e1d5581c/fphar-15-1481273-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9026/11701441/a756ba224190/fphar-15-1481273-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9026/11701441/2a286762f1f3/fphar-15-1481273-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9026/11701441/e56762dde591/fphar-15-1481273-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9026/11701441/4597cc108d34/fphar-15-1481273-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9026/11701441/a0173f9df865/fphar-15-1481273-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9026/11701441/234f0ccf720d/fphar-15-1481273-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9026/11701441/3a59444e1c2b/fphar-15-1481273-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9026/11701441/b37e25c1a567/fphar-15-1481273-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9026/11701441/d3d13eaac6bb/fphar-15-1481273-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9026/11701441/ecd14aa0d3df/fphar-15-1481273-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9026/11701441/ce72e1d5581c/fphar-15-1481273-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9026/11701441/a756ba224190/fphar-15-1481273-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9026/11701441/2a286762f1f3/fphar-15-1481273-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9026/11701441/e56762dde591/fphar-15-1481273-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9026/11701441/4597cc108d34/fphar-15-1481273-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9026/11701441/a0173f9df865/fphar-15-1481273-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9026/11701441/234f0ccf720d/fphar-15-1481273-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9026/11701441/3a59444e1c2b/fphar-15-1481273-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9026/11701441/b37e25c1a567/fphar-15-1481273-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9026/11701441/d3d13eaac6bb/fphar-15-1481273-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9026/11701441/ecd14aa0d3df/fphar-15-1481273-g011.jpg

相似文献

1
Zhili decoction ameliorates ulcerative colitis by modulating gut microbiota and related metabolite, and inhibiting the TLR4/NF-κB/NLRP3 pathway.枳椇子汤通过调节肠道微生物群及其相关代谢产物，并抑制TLR4/NF-κB/NLRP3通路来改善溃疡性结肠炎。
Front Pharmacol. 2024 Dec 23;15:1481273. doi: 10.3389/fphar.2024.1481273. eCollection 2024.
2
Gegen Qinlian decoction ameliorates TNBS-induced ulcerative colitis by regulating Th2/Th1 and Tregs/Th17 cells balance, inhibiting NLRP3 inflammasome activation and reshaping gut microbiota.葛根芩连汤通过调节 Th2/Th1 和 Tregs/Th17 细胞平衡、抑制 NLRP3 炎性小体激活和重塑肠道微生物群来改善 TNBS 诱导的溃疡性结肠炎。
J Ethnopharmacol. 2024 Jun 28;328:117956. doi: 10.1016/j.jep.2024.117956. Epub 2024 Feb 29.
3
Qing-Re-Hua-Shi Decoction ameliorates DSS-induced colitis by modulating multiple signaling pathways and remodeling the gut microbiota and metabolite profile.清热化湿汤通过调节多种信号通路以及重塑肠道微生物群和代谢物谱来改善右旋糖酐硫酸钠诱导的结肠炎。
Front Cell Infect Microbiol. 2025 Apr 2;15:1541289. doi: 10.3389/fcimb.2025.1541289. eCollection 2025.
4
Schisandra chinensis (Turcz.) Baill. Protects against DSS-induced colitis in mice: Involvement of TLR4/NF-κB/NLRP3 inflammasome pathway and gut microbiota.北五味子（Turcz.）Baill. 可预防 DSS 诱导的小鼠结肠炎：涉及 TLR4/NF-κB/NLRP3 炎症小体途径和肠道微生物群。
J Ethnopharmacol. 2022 Nov 15;298:115570. doi: 10.1016/j.jep.2022.115570. Epub 2022 Jul 20.
5
Codonopsis pilosula polysaccharide alleviates ulcerative colitis by modulating gut microbiota and SCFA/GPR/NLRP3 pathway.党参多糖通过调节肠道微生物群和SCFA/GPR/NLRP3通路减轻溃疡性结肠炎。
J Ethnopharmacol. 2025 Jan 30;337(Pt 2):118928. doi: 10.1016/j.jep.2024.118928. Epub 2024 Oct 10.
6
Buyang Huanwu Decoction stabilizes atherosclerotic vulnerable plaques by regulating intestinal flora, TLR4-NF-κB-NLRP3 inflammatory pathway and mitophagy.补阳还五汤通过调节肠道菌群、TLR4-NF-κB-NLRP3炎症通路和线粒体自噬来稳定动脉粥样硬化易损斑块。
Phytomedicine. 2025 Jul;142:156751. doi: 10.1016/j.phymed.2025.156751. Epub 2025 Apr 12.
7
Ganluyin ameliorates DSS-induced ulcerative colitis by inhibiting the enteric-origin LPS/TLR4/NF-κB pathway.甘露饮通过抑制肠源性 LPS/TLR4/NF-κB 通路改善 DSS 诱导的溃疡性结肠炎。
J Ethnopharmacol. 2022 May 10;289:115001. doi: 10.1016/j.jep.2022.115001. Epub 2022 Jan 24.
8
Banxia Xiexin decoction modulates gut microbiota and gut microbiota metabolism to alleviate DSS-induced ulcerative colitis.半夏泻心汤通过调节肠道微生物群及其代谢物缓解 DSS 诱导的溃疡性结肠炎。
J Ethnopharmacol. 2024 May 23;326:117990. doi: 10.1016/j.jep.2024.117990. Epub 2024 Feb 27.
9
suppresses gut microbiota related TNF inflammatory pathway to alleviates ulcerative colitis.抑制肠道微生物群相关的肿瘤坏死因子炎症通路以减轻溃疡性结肠炎。
Front Immunol. 2025 Apr 22;16:1537325. doi: 10.3389/fimmu.2025.1537325. eCollection 2025.
10
Huangqin Decoction ameliorates ulcerative colitis by regulating fatty acid metabolism to mediate macrophage polarization via activating FFAR4-AMPK-PPARα pathway.黄芩汤通过激活FFAR4-AMPK-PPARα通路调节脂肪酸代谢以介导巨噬细胞极化，从而改善溃疡性结肠炎。
J Ethnopharmacol. 2023 Jul 15;311:116430. doi: 10.1016/j.jep.2023.116430. Epub 2023 Mar 28.

本文引用的文献

1
Berberine regulates intestinal microbiome and metabolism homeostasis to treat ulcerative colitis.小檗碱通过调节肠道微生物组和代谢稳态治疗溃疡性结肠炎。
Life Sci. 2024 Feb 1;338:122385. doi: 10.1016/j.lfs.2023.122385. Epub 2024 Jan 4.
2
Scytosiphon lomentaria fucoidan ameliorates DSS-induced colitis in dietary fiber-deficient mice via modulating the gut microbiota and inhibiting the TLR4/NF-κB/MLCK pathway.石花菜岩藻聚糖硫酸酯通过调节肠道微生物群和抑制 TLR4/NF-κB/MLCK 通路改善膳食纤维缺乏小鼠的 DSS 诱导的结肠炎。
Int J Biol Macromol. 2023 Dec 31;253(Pt 8):127337. doi: 10.1016/j.ijbiomac.2023.127337. Epub 2023 Oct 31.
3
The role of Lactobacillus in inflammatory bowel disease: from actualities to prospects.
乳酸杆菌在炎症性肠病中的作用：从现状到前景
Cell Death Discov. 2023 Sep 29;9(1):361. doi: 10.1038/s41420-023-01666-w.
4
Interactions between gut microbiota and polyphenols: A mechanistic and metabolomic review.肠道微生物群与多酚的相互作用：机制和代谢组学综述。
Phytomedicine. 2023 Oct;119:154979. doi: 10.1016/j.phymed.2023.154979. Epub 2023 Jul 18.
5
L. Extract Attenuates DSS-Induced Ulcerative Colitis in Mice by Regulating Inflammatory Factor Release and Blocking NF-B Activation.L. 通过调节炎症因子释放和抑制 NF-B 激活来减轻 DSS 诱导的小鼠溃疡性结肠炎。
J Med Food. 2023 Sep;26(9):663-671. doi: 10.1089/jmf.2023.K.0037. Epub 2023 Aug 2.
6
Inflammatory Bowel Diseases and Gut Microbiota.炎症性肠病与肠道微生物群。
Int J Mol Sci. 2023 Feb 14;24(4):3817. doi: 10.3390/ijms24043817.
7
Quercetin effectively improves LPS-induced intestinal inflammation, pyroptosis, and disruption of the barrier function through the TLR4/NF-κB/NLRP3 signaling pathway and .槲皮素通过TLR4/NF-κB/NLRP3信号通路有效改善脂多糖诱导的肠道炎症、细胞焦亡及屏障功能破坏。
Food Nutr Res. 2022 Dec 30;66. doi: 10.29219/fnr.v66.8948. eCollection 2022.
8
Flavor production in fermented chayote inoculated with lactic acid bacteria strains: Genomics and metabolomics based analysis.发酵佛手瓜接种乳酸菌产生风味的研究：基于基因组学和代谢组学的分析。
Food Res Int. 2023 Jan;163:112224. doi: 10.1016/j.foodres.2022.112224. Epub 2022 Nov 24.
9
Tea (Camellia sinensis) ameliorates DSS-induced colitis and liver injury by inhibiting TLR4/NF-κB/NLRP3 inflammasome in mice.茶（Camellia sinensis）通过抑制 TLR4/NF-κB/NLRP3 炎性小体减轻小鼠 DSS 诱导的结肠炎和肝损伤。
Biomed Pharmacother. 2023 Feb;158:114136. doi: 10.1016/j.biopha.2022.114136. Epub 2022 Dec 17.
10
Integrated Metabonomics and Network Pharmacology to Reveal the Action Mechanism Effect of Shaoyao Decoction on Ulcerative Colitis.基于代谢组学和网络药理学探讨芍药甘草汤治疗溃疡性结肠炎的作用机制
Drug Des Devel Ther. 2022 Oct 27;16:3739-3776. doi: 10.2147/DDDT.S375281. eCollection 2022.