Acquaticci Laura, Santanatoglia Agnese, Vittadini Elena, Beghelli Daniela, La Terza Antonietta, Zengin Gokhan, Caprioli Giovanni
Chemistry Interdisciplinary Project (ChIP), School of Pharmacy, University of Camerino, Via Madonna delle Carceri 9/B, 62032 Camerino, Italy.
School of Biosciences and Veterinary Medicine, University of Camerino, Via Gentile III da Varano, 62032 Camerino, Italy.
Antioxidants (Basel). 2024 Dec 13;13(12):1526. doi: 10.3390/antiox13121526.
This study provides the first comprehensive evaluation of the bioactive potential of acorn flour extracts (, Fagaceae) prepared at different temperatures (20, 60, 80 and 100 °C), focusing on polyphenolic content, antioxidant properties and enzyme inhibitory activities. Through HPLC-ESI-MS/MS analysis, 36 bioactive compounds were identified, with the extract at 60 °C showing the highest concentrations of key polyphenols, notably gallic acid (210,008.9 mg·kg) and ellagic acid (45,469.6 mg·kg). This extract also exhibited a high antioxidant activity and significant inhibition of glucosidase and acetylcholinesterase, suggesting potential benefits for diabetes management and neuroprotection. The results indicate that extraction temperature affects bioactivity, with the 60 °C extract standing out as a promising candidate for nutraceutical, pharmaceutical, and cosmeceutical applications due to its rich polyphenol profile and potent biological properties.
本研究首次全面评估了在不同温度（20、60、80和100°C）下制备的橡实粉提取物（壳斗科）的生物活性潜力，重点关注多酚含量、抗氧化性能和酶抑制活性。通过HPLC-ESI-MS/MS分析，鉴定出36种生物活性化合物，60°C下的提取物中关键多酚的浓度最高，尤其是没食子酸（210,008.9毫克·千克）和鞣花酸（45,469.6毫克·千克）。该提取物还表现出高抗氧化活性，并对葡萄糖苷酶和乙酰胆碱酯酶有显著抑制作用，表明对糖尿病管理和神经保护具有潜在益处。结果表明，提取温度会影响生物活性，60°C的提取物因其丰富的多酚谱和强大的生物学特性，成为营养保健品、药品和化妆品应用的有前景的候选物。
