Laboratory of Management and Valorization of Forest Resources, National Researches Institute of Rural engineering, Water and Forests (INRGREF), University of Carthage, Av. Hedi Karray, BP 10 2080-, Ariana., Tunisia.
Department of Biology, Faculty of Sciences of Tunis, University of Tunis El ManarII, 2092, Tunis, Tunisia.
Chem Biodivers. 2023 May;20(5):e202300047. doi: 10.1002/cbdv.202300047. Epub 2023 Apr 4.
The current study was conducted to investigate the chemical composition of Quercus canariensis flour acorns extracts as well as its biological activities in regards to the growing area using spectrophotometric and chromatographic techniques. The phenolic profile was composed of 19 compounds identified through HPLC-DAD analysis. Coumarin was the most abundant compound quantified in BniMtir, Nefza and ElGhorra and gallic (12.58-20.52 %), syringic (4.70-7.64 %) and trans-ferulic (2.28-2.94 %) acids were the abundant phenolic acids while kaempferol was the major flavonoid compounds quantified only in Quercus canariensis growing in BniMtir. On the other hand, Ain Snoussi acorn extract was characterized by its high content in luteolin-7-O-glucoside (58.46 %). The in-vitro antioxidant activities of the studied extracts were investigated and the results showed that Nefza ethanolic extract's has the highest activities. A bactericidal effect against Staphylococcus aureus was observed only by Elghorra population. On the other hand, Ain Snoussi acorn extract was efficient to inhibit growth of pathogenic bacteria, mentioned the highest activity against Escherichia coli. These results is the first study highlighted that zeen oak acorns are an excellent source of natural antioxidants and antibacterial compounds related to their lysozyme activity which could be exploited in the pharmaceutical and food sectors.
本研究旨在使用分光光度法和色谱技术，调查 Quercus canariensis 面粉橡实提取物的化学成分及其生物活性与生长区域的关系。通过 HPLC-DAD 分析鉴定出 19 种化合物组成了酚类成分。香豆素是在 BniMtir、Nefza 和 ElGhorra 中含量最丰富的化合物，没食子酸（12.58-20.52）、丁香酸（4.70-7.64）和反式阿魏酸（2.28-2.94）是含量丰富的酚酸，而山奈酚则是仅在 BniMtir 生长的 Quercus canariensis 中含量最丰富的黄酮类化合物。另一方面，Ain Snoussi 橡实提取物的特征是其木犀草素-7-O-葡萄糖苷含量较高（58.46）。研究了所测提取物的体外抗氧化活性，结果表明，Nefza 乙醇提取物的活性最高。仅 Elghorra 种群对金黄色葡萄球菌表现出杀菌作用。另一方面，Ain Snoussi 橡实提取物能有效抑制病原菌的生长，对大肠杆菌的活性最高。这些结果首次表明，欧洲栓皮栎橡子是天然抗氧化剂和抗菌化合物的极好来源，与它们的溶菌酶活性有关，可在制药和食品领域得到利用。
