Monitoring of Antibiotic Resistance Patterns Within Al-Karak Governmental Hospital, Jordan, in 2022.

: Antimicrobial resistance is considered one of the foremost global public health challenges, and its prevalence is increasing. In Jordan, particularly in Al-Karak Governorate, there is a lack of sufficient data on antimicrobial resistance to make accurate assessments. The main aim of the current study was to evaluate antibiotic resistance trends in clinical specimens from 2022 and assess antibiotic resistance patterns. The emphasis on the WHO antibiotic classification as Access, Watch, and Reserved (AWaRe) was adopted in the current study. : Among Gram-positive bacteria, exhibited 100% susceptibility to nitrofurantoin and 96% to vancomycin, exhibited 100% susceptibility to teicoplanin, while CoNS (coagulase-negative ) showed moderate resistance to Trimethoprim + Sulfamethoxazole (63%) and clindamycin (47%). Among Gram-negative bacteria, and displayed high susceptibility to fosfomycin (: 95%, : 80%) and amikacin (: 93%, : 81%). Resistance was notable for trimethoprim + sulfamethoxazole (: 47%, : 53%) and nitrofurantoin (: 30%). exhibited the highest proportion of XDR strains (15%), followed by (11%) and (4%), while PDR strains were found in (6%), (3%), and (0.6%). XDR was observed in 4% of CoNS and 3% of (α), with exhibiting both XDR and PDR at 1%. : A cross-sectional retrospective study of bacterial species and their antimicrobial susceptibility was carried out at a hospital in Al Karak, Jordan, from January to December of 2022, the study included 1187 isolates from all locations in Al-Karak Governmental Hospital. : The significant prevalence of XDR and PDR strains in key pathogens, particularly and , underscores the need for a robust Antimicrobial Stewardship Program (ASP) and infection control measures at Al-Karak Governmental Hospital. High susceptibility in several Access group antibiotics (e.g., amikacin and nitrofurantoin) supports their prioritization in empirical therapy, while the emergence of resistance in Watch and Reserved antibiotics highlights the necessity for rational use. These findings are very important for adjusting the local strategies to lower the spread of resistant strains and improve clinical outcomes.