Liu Simeng, He Mingang, Sun Hefen, Wu Yi, Jin Wei
Key Laboratory of Breast Cancer in Shanghai, Fudan University Shanghai Cancer Center, Shanghai 200032, China.
Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China.
Genes (Basel). 2024 Nov 28;15(12):1541. doi: 10.3390/genes15121541.
Firstly, 5-hydroxytryptamine G-protein-coupled receptors () are a family of 13 genes associated with cancer progression. Nevertheless, a comprehensive understanding of in cancer remains largely lacking.
We tested the gene expression levels and prognostic values for the in relation to pan-cancer. A subsequent analysis examined the relationships among expression and clinical characteristics, immune subtypes, stemness scores, tumor microenvironments (TMEs), single-cell analyses, and drug sensitivity.
A significant difference in expression was found between normal tissues and tumors. expressed higher levels in breast invasive carcinoma (BRCA), colon adenocarcinoma, and liver hepatocellular carcinoma. gene expression was correlated with prognosis in many cancers. were associated with poorer overall survival for head and neck squamous cell carcinoma. In addition, expression correlated significantly with the stemness scores of RNA and DNA, TMB, and MSI, as well as stromal and immune scores of pan-cancer patients. Additionally, the expression of was correlated significantly with immune cells and immune checkpoint genes in a variety of cancers, such as BRCA, brain lower-grade glioma, and lung adenocarcinoma. Immune regulation and TME were both regulated by . Using single-cell analysis, we found that the gene set of correlated with many cancer-related functional states in retinoblastoma. Moreover, drug sensitivity and were significantly correlated. Furthermore, we validated results in breast cancer and found knockdown of inhibited breast cancer cell growth and metastasis.
As prognostic indicators, hold considerable promise and offer insights into the therapeutic targets for malignancy.
首先，5-羟色胺G蛋白偶联受体（）是与癌症进展相关的13个基因家族。然而，对其在癌症中的全面理解仍然非常缺乏。
我们测试了该基因在泛癌中的表达水平和预后价值。随后的分析研究了该基因表达与临床特征、免疫亚型、干性评分、肿瘤微环境（TME）、单细胞分析和药物敏感性之间的关系。
在正常组织和肿瘤之间发现了该基因表达的显著差异。在乳腺浸润性癌（BRCA）、结肠腺癌和肝细胞癌中表达水平较高。该基因的表达在许多癌症中与预后相关。在头颈部鳞状细胞癌中，其与较差的总生存期相关。此外，该基因表达与RNA和DNA的干性评分、肿瘤突变负荷（TMB）和微卫星不稳定性（MSI）以及泛癌患者的基质和免疫评分显著相关。此外，在多种癌症中，如BRCA、脑低级别胶质瘤和肺腺癌，该基因的表达与免疫细胞和免疫检查点基因显著相关。免疫调节和TME均受该基因调控。通过单细胞分析，我们发现该基因集与视网膜母细胞瘤中许多癌症相关的功能状态相关。此外，药物敏感性与该基因显著相关。此外，我们在乳腺癌中验证了结果，发现敲低该基因可抑制乳腺癌细胞的生长和转移。
作为预后指标，该基因具有很大的前景，并为恶性肿瘤的治疗靶点提供了见解。
