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全基因组关联研究揭示了萨亚尔卡肥尾粗毛绵羊品种肉用性能的几个候选基因。

Genome-Wide Association Studies Revealed Several Candidate Genes of Meat Productivity in Saryarka Fat-Tailed Coarse-Wool Sheep Breed.

作者信息

Dossybayev Kairat, Amandykova Makpal, Orakbayeva Ainur, Adylkanova Sholpan, Kozhakhmet Altynay, Yergali Kanagat, Kulboldin Temirlan, Kulataev Beibit, Torekhanov Aibyn

机构信息

LLP «Kazakh Research Institute of Animal Husbandry and Fodder Production», 51 Zhandosov Street, Almaty 050071, Kazakhstan.

Faculty of Veterinary Medicine and Zooengineering, Kazakh National Agrarian Research University, 8 Abai Avenue, Almaty 050010, Kazakhstan.

出版信息

Genes (Basel). 2024 Nov 29;15(12):1549. doi: 10.3390/genes15121549.

DOI:10.3390/genes15121549

PMID:39766815

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11728008/

Abstract

BACKGROUND

Saryarka sheep belong to fat-tailed coarse-wool sheep breed. This breed is distinguished by increased meat productivity while being competitive in young lamb production. Live weight and body indices are relevant data for assessing sheep body constitution, which directly affects the breeding characteristics and meat productivity of animals.

OBJECTIVES

This study aimed to find associations with SNPs and nine phenotypic characteristics of the Saryarka fat-tailed coarse-wool sheep breed including live weight and eight body indices (wither height, rump height, bicoastal diameter, body depth, body length, rump width, heart girth, and cannon bone circumference), and find candidate genes related to these characteristics.

METHODS

A total of 100 animals from the Karaganda region of Kazakhstan were used in this study. Live weight and eight body indices of sheep were measured using tape and electronic scales. The blood samples of the animals were used for DNA extraction. DNA samples were genotyped with the OvineSNP50 Genotyping BeadChip and analyzed using GWAS. Statistically significant SNPs were identified for each characteristic trait referencing the genome of (Oar_v3.1) using BioMart.

RESULTS

The GWAS results demonstrated a substantial chromosomal-level correlation between 32 chromosome-wide significant and suggestively significant SNPs in the studied sheep breed. Overall, seven SNPs located in seven different genes were revealed as candidates for live weight and four body indices: s20793.1 SNP in the gene for live weight, OAR4_54217431.1 SNP in the gene for bicoastal diameter, s25229.1 in the gene, and s01175.1 SNP in the gene for rump width, OAR2_175574781.1 SNP in the gene for heart girth, and OAR1_209022621.1 SNP in the gene and s15415.1 SNP in the gene for cannon bone circumference. Some of these genes were previously reported to be involved in body constitution and fat deposit in other sheep breeds.

CONCLUSIONS

The results of the present study open up new opportunities for targeted sheep breeding for meat and fat productivity.

摘要

背景

萨雷阿尔卡羊属于肥尾粗毛羊品种。该品种的特点是肉用生产力提高，在羔羊生产方面具有竞争力。体重和体尺指标是评估羊体质的相关数据，直接影响动物的繁殖特性和肉用生产力。

目的

本研究旨在寻找与萨雷阿尔卡肥尾粗毛羊品种的单核苷酸多态性（SNPs）和九个表型特征（包括体重和八个体尺指标：鬐甲高、臀高、体宽、体深、体长、臀宽、胸围和管围）之间的关联，并找出与这些特征相关的候选基因。

方法

本研究共使用了来自哈萨克斯坦卡拉干达地区的100只羊。使用卷尺和电子秤测量羊的体重和八个体尺指标。采集动物的血液样本用于DNA提取。DNA样本用绵羊50K基因分型芯片进行基因分型，并使用全基因组关联研究（GWAS）进行分析。利用BioMart，参照（Oar_v3.1）基因组，为每个特征性状鉴定出具有统计学意义的SNPs。

结果

GWAS结果表明，在所研究的绵羊品种中，32个全染色体显著和暗示性显著的SNPs之间存在显著的染色体水平相关性。总体而言，位于七个不同基因中的七个SNPs被确定为体重和四个体尺指标的候选基因：体重基因中的s20793.1 SNP、体宽基因中的OAR4_54217431.1 SNP、某基因中的s25229.1、臀宽基因中的s01175.1 SNP、胸围基因中的OAR2_175574781.1 SNP、管围基因中的OAR1_209022621.1 SNP和某基因中的s15415.1 SNP。其中一些基因先前在其他绵羊品种中被报道与体质和脂肪沉积有关。