Ma Changlu, Xu Chen, Zheng Mumin, Zhang Shuwen, Liu Qifeng, Lyu Jiaping, Pang Xiaoyang, Wang Yinghong
Department of Food and Bio-Engineering, Beijing Vocational College of Agriculture, Beijing 102442, China.
Institute of Food Science and Technology, Chinese Academy of Agricultural Science, Beijing 100193, China.
Foods. 2024 Dec 16;13(24):4058. doi: 10.3390/foods13244058.
Hyperlipidemia poses significant risks for cardiovascular diseases, with emerging evidence underscoring the critical role of gut microbiota in metabolic regulation. This study explores CAAS36, a probiotic strain with promising cholesterol-lowering capabilities, assessing its impact on hyperlipidemic hamsters. Utilizing 1H NMR-based metabolomics and 16S rRNA gene sequencing, we observed that CAAS36 treatment not only altered metabolic pathways but also reshaped gut microbiota composition. Notably, the treatment restored the balance between Firmicutes and Bacteroidetes and significantly increased the abundance of propionate-producing Muribaculaceae. Metabolically, CAAS36 administration led to the normalization of key lipid markers, including reductions in total cholesterol, LDL-C, and triglycerides (29.9%, 29.4% and 32.6%), while enhancing the protective HDL-C levels. These effects were accompanied by significant increases in beneficial metabolites such as propionate and succinate, which are known for their roles in preventing metabolic disorders. These findings highlight the dual regulatory effects of CAAS36 on the metabolic profile and gut microbiota, suggesting a substantial potential for this probiotic in the management of hyperlipidemia and possibly other metabolic diseases. Future applications may include its use as a natural therapeutic agent in clinical settings, aiming to reduce reliance on conventional pharmaceuticals and their associated side effects.
高脂血症对心血管疾病构成重大风险，新出现的证据强调了肠道微生物群在代谢调节中的关键作用。本研究探索了具有显著降胆固醇能力的益生菌菌株CAAS36，评估其对高脂血症仓鼠的影响。利用基于1H NMR的代谢组学和16S rRNA基因测序，我们观察到CAAS36治疗不仅改变了代谢途径，还重塑了肠道微生物群组成。值得注意的是，该治疗恢复了厚壁菌门和拟杆菌门之间的平衡，并显著增加了产生丙酸盐的毛螺菌科的丰度。在代谢方面，给予CAAS36导致关键脂质标志物正常化，包括总胆固醇、低密度脂蛋白胆固醇和甘油三酯降低（分别为29.9%、29.4%和32.6%），同时提高了具有保护作用的高密度脂蛋白胆固醇水平。这些作用伴随着有益代谢物如丙酸盐和琥珀酸盐的显著增加，它们在预防代谢紊乱中发挥作用。这些发现突出了CAAS36对代谢谱和肠道微生物群的双重调节作用，表明这种益生菌在高脂血症以及可能的其他代谢疾病管理中具有巨大潜力。未来的应用可能包括将其用作临床环境中的天然治疗剂，旨在减少对传统药物及其相关副作用的依赖。
