Lima Karen Rodrigues, Tavares Hugo Giordano, Pereira Ramona Ramalho de Souza, Carvalho Jaqueline do Carmo Lima, Botelho Roberta de Oliveira, Reis Spuri Aline Chaves, Dobbss Leonardo Barros, Machado Alan Rodrigues Teixeira, Orlando Débora Ribeiro, Remédio Rafael Neodini, Paiva Saul Martins de, Moura Rodrigo Ferreira de, Dias-Peixoto Marco Fabrício, Pereira Luciano José, Andrade Eric Francelino
Department of Health Sciences, Universidade Federal de Lavras (UFLA), Lavras 37200-000, MG, Brazil.
Postgraduate Program in Health Sciences (PPGCS), Federal University of the Jequitinhonha and Mucuri Valleys (UFVJM), Diamantina 39803-371, MG, Brazil.
Biomedicines. 2024 Nov 27;12(12):2710. doi: 10.3390/biomedicines12122710.
Periodontal disease (PD) leads to the destruction of supportive tissues through an inflammatory response induced by biofilm accumulation. This low-grade systemic inflammation from PD increases the risk of comorbidities. Among potential therapeutic agents for PD, humic acids (HAs) are notable for their anti-inflammatory and immunomodulatory properties. This study aimed to evaluate the effects of varying HA doses on PD progression in an experimental model. Fifty-four Wistar rats were assigned to six groups (n = 8 each): control, PD, PD + 40 mg/kg HA, PD + 80 mg/kg HA, PD + 160 mg/kg HA, and PD + 320 mg/kg HA. HA from vermicompost was administered daily by gavage for 28 days, with PD induced by ligature on day 14. Post-euthanasia, mandibular samples were analyzed histomorphometrically for bone loss and osteocyte density. Alveolar bone topography and elemental composition were examined using Scanning Electron Microscopy (SEM) coupled with Energy Dispersive Spectroscopy (EDS). Renal and hepatic tissues were assessed histopathologically. Data were analyzed with Analysis of Variance (ANOVA) and Duncan's test. HA-treated animals showed reduced epithelial attachment loss and alveolar bone loss, with improved bone quality parameters, such as reduced pore number and diameter and increased osteocyte density compared to the PD group. Renal lesions observed in PD animals were mitigated at 40 and 80 mg/kg HA doses. HA treatment improves alveolar bone integrity and, at lower doses, reduces PD-induced renal lesions.
牙周病（PD）通过生物膜积累引发的炎症反应导致支持组织的破坏。PD引发的这种低度全身炎症会增加患合并症的风险。在PD的潜在治疗药物中，腐殖酸（HA）因其抗炎和免疫调节特性而备受关注。本研究旨在评估不同剂量的HA对实验模型中PD进展的影响。将54只Wistar大鼠分为六组（每组n = 8）：对照组、PD组、PD + 40 mg/kg HA组、PD + 80 mg/kg HA组、PD + 160 mg/kg HA组和PD + 320 mg/kg HA组。通过灌胃每日给予来自蚯蚓堆肥的HA，持续28天，在第14天通过结扎诱导PD。安乐死后，对下颌样本进行组织形态计量学分析，以评估骨丢失和骨细胞密度。使用扫描电子显微镜（SEM）结合能量色散光谱（EDS）检查牙槽骨的地形和元素组成。对肾和肝组织进行组织病理学评估。数据采用方差分析（ANOVA）和邓肯检验进行分析。与PD组相比，HA治疗的动物上皮附着丧失和牙槽骨丢失减少，骨质量参数得到改善，如孔隙数量和直径减少，骨细胞密度增加。在40和80 mg/kg HA剂量下，PD动物中观察到的肾损伤得到缓解。HA治疗可改善牙槽骨完整性，且在较低剂量下可减少PD诱导的肾损伤。
