Orlando Patrícia Ribeiro, Tavares Hugo Giordano, de Souza Pereira Ramona Ramalho, Silva Gabriela, do Carmo Lima Carvalho Jaqueline, Machado Alan Rodrigues Teixeira, Dobbss Leonardo Barros, Peixoto Marco Fabrício Dias, Pereira Luciano Jose, Andrade Eric Francelino
Health Sciences Program, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Diamantina, Brazil.
Department of Exact Sciences, Universidade do Estado de Minas Gerais, João Monlevade, Minas Gerais, 35930-314, Brazil.
Curr Top Med Chem. 2025;25(5):645-656. doi: 10.2174/0115680266302476240510115556.
Humic acid (HA) is a bioproduct that can be extracted from different sources and has anti-inflammatory properties that have been little explored in the treatment and prevention of Periodontal Disease (PD). Thus, we aimed to investigate the effects of oral administration of HA on the progression of PD in rats.
Twenty-four male Wistar rats were distributed into three experimental groups (Control/ Sham, PD, and PD + HA). HA was administered by gavage (80 mg/kg/day) for 28 days, and PD was induced 14 days after the beginning of treatment. Bone loss, bone topography, and surface elemental composition were analyzed. Circulating IL1-beta, TNF-alpha, and IL-10 levels were evaluated through Enzyme-Linked Immunosorbent Assay (ELISA).
The animals treated with HA showed lower bone loss (p < 0.05). Calcium and phosphorus levels on the alveolar bone surface were lower in the PD group (p < 0.05) compared to the control group, whereas the animals treated with HA exhibited attenuation in this loss (p < 0.05). The animals treated with HA showed reduced TNF-alpha, IL1-beta, IL-10, and the TNF-alpha/IL-10 ratio compared to those with PD (p < 0.05).
Treatment with HA attenuated the parameters of alveolar bone loss and modulated systemic inflammatory parameters in rats with ligature-induced PD.
腐殖酸（HA）是一种可从不同来源提取的生物制品，其抗炎特性在牙周病（PD）的治疗和预防方面鲜有研究。因此，我们旨在研究口服HA对大鼠牙周病进展的影响。
将24只雄性Wistar大鼠分为三个实验组（对照组/假手术组、牙周病组和牙周病+腐殖酸组）。通过灌胃给予腐殖酸（80毫克/千克/天），持续28天，在治疗开始14天后诱导牙周病。分析骨丢失、骨形态和表面元素组成。通过酶联免疫吸附测定（ELISA）评估循环中的白细胞介素-1β、肿瘤坏死因子-α和白细胞介素-10水平。
接受腐殖酸治疗的动物骨丢失较少（p<0.05）。与对照组相比，牙周病组牙槽骨表面的钙和磷水平较低（p<0.05），而接受腐殖酸治疗的动物这种丢失有所减轻（p<0.05）。与牙周病组动物相比，接受腐殖酸治疗的动物肿瘤坏死因子-α、白细胞介素-1β、白细胞介素-10以及肿瘤坏死因子-α/白细胞介素-10比值均降低（p<0.05）。
在结扎诱导的大鼠牙周病模型中，腐殖酸治疗减轻了牙槽骨丢失参数并调节了全身炎症参数。
