Robinson J Paul
Purdue University, West Lafayette, Indiana, USA.
Curr Protoc Cytom. 2005 Feb;Chapter 12:Unit 12.1. doi: 10.1002/0471142956.cy1201s31.
This unit considers the issues of which tool to use--flow cytometry or imaging--and under what conditions. In particular, it compares the advantages and disadvantages of flow and image cytometry and provides examples illustrating the proper choice of each technology. The result is a better understanding of why the two technologies are complementary in many applications. It is clear that many scientists use the tools that are familiar to them, often in preference to the best tool. In cases where very advanced and rather expensive technologies are concerned, this is not surprising. However, there are clearly times when one form of cytometry is definitely superior to another. What then constitute the criteria for a decision when both flow cytometry and imaging are available? This unit addresses some of these concerns.
本单元将探讨使用哪种工具（流式细胞术还是成像技术）以及在何种条件下使用的问题。具体而言，它比较了流式细胞术和成像细胞术的优缺点，并提供了说明每种技术正确选择的示例。结果是能更好地理解为何这两种技术在许多应用中具有互补性。很明显，许多科学家使用他们熟悉的工具，往往优先于最佳工具。在涉及非常先进且相当昂贵的技术的情况下，这并不奇怪。然而，显然有时一种细胞术形式绝对优于另一种。那么，当流式细胞术和成像技术都可用时，做出决策的标准是什么呢？本单元将解决其中一些问题。
