Zhang Yu, Li Yanyan, Han Haibin, Wang Xiaoling, Gao Shujing, Zhao Qing, Bieerdebieke Halima, Xu Linbo, Zang Qicong, Wang Hui, Bai Penghua, Lin Kejian
Key Laboratory of Biohazard Monitoring, Green Prevention and Control for Artificial Grassland, Ministry of Agriculture and Rural Affairs, Institute of Grassland Research of Chinese Academy of Agricultural Sciences, Hohhot 010010, China.
Research Center for Grassland Entomology, Inner Mongolia Agricultural University, Hohhot 010020, China.
Life (Basel). 2024 Dec 23;14(12):1705. doi: 10.3390/life14121705.
The beet webworm, , is a typical migratory pest. Although miRNAs participate in many physiological functions, little is known about the functions of miRNAs in olfactory regulation. In this study, 1120 (869 known and 251 novel) miRNAs were identified in the antennae of by using high-throughput sequencing technology. Among the known miRNAs, 189 from 49 families were insect-specific, indicating that these miRNAs might play unique roles in insects. Furthermore, based on the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses, we found that 3647 and 1393 miRNAs were associated with localization and the regulation of localization, respectively, and 80 miRNAs were enriched in the neuroactive ligand-receptor interaction pathway. These miRNAs might be involved in the olfactory system of . Notably, qRT-PCR showed that most of the tested miRNAs presented similar expression patterns compared with the RNA-seq data and that , , and were significantly differentially expressed in the antennae of males and females. In addition, 21 miRNAs were predicted to target 23 olfactory genes, including 10 odorant-binding proteins (OBPs), 3 chemosensory proteins (CSPs), 4 odorant receptors (ORs), 1 ionotropic receptor (IR), and 5 gustatory receptors (GRs). The olfactory-related miRNAs exhibited low-abundance transcripts, except and , and gender-biased expression was not observed for olfactory-related miRNAs. Our findings provide an overview of the potential miRNAs involved in olfactory regulation, which may provide important information on the function of miRNAs in the insect olfactory system.
甜菜夜蛾是一种典型的迁飞性害虫。尽管微小RNA（miRNA）参与许多生理功能，但关于miRNA在嗅觉调节中的功能却知之甚少。在本研究中，通过高通量测序技术在甜菜夜蛾触角中鉴定出1120个miRNA（869个已知miRNA和251个新miRNA）。在已知的miRNA中，来自49个家族的189个是昆虫特异性的，这表明这些miRNA可能在昆虫中发挥独特作用。此外，基于基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析，我们发现分别有3647个和1393个miRNA与定位和定位调节相关，并且有80个miRNA在神经活性配体-受体相互作用途径中富集。这些miRNA可能参与甜菜夜蛾的嗅觉系统。值得注意的是，定量逆转录聚合酶链反应（qRT-PCR）表明，与RNA测序数据相比，大多数测试的miRNA呈现出相似的表达模式，并且在雄性和雌性甜菜夜蛾的触角中，某些miRNA存在显著差异表达。此外，预测有21个miRNA靶向23个嗅觉基因，包括10个气味结合蛋白（OBP）、3个化学感受蛋白（CSP）、4个气味受体（OR）、1个离子型受体（IR）和5个味觉受体（GR）。除了某些miRNA外，嗅觉相关的miRNA表现出低丰度转录本，并且未观察到嗅觉相关miRNA的性别偏向性表达。我们的研究结果概述了参与嗅觉调节的潜在miRNA，这可能为miRNA在昆虫嗅觉系统中的功能提供重要信息。
