Bugelski P J, Kirsh R L, Sowinski J M, Poste G
Am J Pathol. 1985 Mar;118(3):419-24.
The macrophage content of experimental B16 melanoma metastases at different stages of their growth has been quantified with the use of morphometry in conjunction with a recently developed histochemical method for selectively staining intratumoral macrophages. Data are presented from analyses of 954 sections of 155 individual lung metastases, showing that the macrophage content of individual B16 melanoma lung metastases not only varies significantly but also falls dramatically once metastases contain more than 700 tumor cells. In addition to providing new information on host response reactions of micrometastases, these experiments also indicate that conclusions on intratumoral macrophages derived from studies of large primary tumors and metastases in advanced stages of growth may have little or no relevance to events in micrometastases.
利用形态测量法结合最近开发的一种选择性染色肿瘤内巨噬细胞的组织化学方法，对实验性B16黑色素瘤转移灶在不同生长阶段的巨噬细胞含量进行了定量分析。本文给出了对155个个体肺转移灶的954个切片的分析数据，结果表明，单个B16黑色素瘤肺转移灶的巨噬细胞含量不仅差异显著，而且一旦转移灶包含超过700个肿瘤细胞，其巨噬细胞含量就会急剧下降。这些实验除了提供关于微转移灶宿主反应的新信息外，还表明，从大型原发性肿瘤和生长晚期转移灶的研究中得出的关于肿瘤内巨噬细胞的结论，可能与微转移灶中的情况几乎没有关联或完全无关。
