Zhao Mengjie, Zhou Gaoxing, Liu Peigang, Wang Zhifeng, Yang Lu, Li Tianyan, Tojiddinovich Valiev Sayfiddin, Ubaydullayevich Nasirillayev Bakhtiyar, Adilovna Ismatullaeva Diloram, Khasanboy Ugl Khudjamatov Safarali, Liu Yan, Lv Zhiqiang, Wei Jia, Lin Tianbao
Institute of Sericulture and Tea, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
Key Laboratory of Forest Resources and Utilization in Xinjiang of National Forestry and Grassland Administration, Key Laboratory of Fruit Tree Species Breeding and Cultivation, Xinjiang Academy of Forestry, Urumqi 830052, China.
Int J Mol Sci. 2024 Dec 12;25(24):13341. doi: 10.3390/ijms252413341.
Bud dormancy is a critical adaptive trait in woody plants, essential for enduring harsh winter conditions. The relationship between bud break timing and cold resistance is complex and has been a subject of debate. This study utilizes a Genome-Wide Association Study (GWAS) on 201 natural mulberry populations to identify the gene, which shows the strongest association with bud break timing. Known for its role in cold resistance, 's link to bud break timing suggests a direct correlation between these traits. Expression analysis of in mulberry trees indicates peak activity in dormant buds, declining as dormancy ends. Selective sweep analysis on germplasms from contrasting climates reveals positive selection in in cold-resistant Uzbek germplasms. Overexpression of in early-budding germplasms significantly delays bud break, confirming its regulatory role. These findings highlight as a key determinant of cold tolerance variability among mulberry germplasms, with its expression directly correlated with bud break timing. This provides a molecular basis for selecting cold-resistant mulberry germplasms based on bud break timing in breeding programs.
芽休眠是木本植物的一种关键适应性特征，对于耐受严酷的冬季条件至关重要。芽萌发时间与抗寒性之间的关系复杂，一直是争论的焦点。本研究对201个天然桑树种群进行全基因组关联研究（GWAS），以鉴定与芽萌发时间关联最强的基因。因其在抗寒中的作用而闻名，它与芽萌发时间的联系表明这些性状之间存在直接关联。桑树中该基因的表达分析表明，其在休眠芽中活性最高，随着休眠结束而下降。对来自不同气候的种质进行的选择性扫描分析显示，在耐寒的乌兹别克种质中该基因受到正选择。在早萌发芽种中过表达该基因可显著延迟芽萌发，证实了其调控作用。这些发现突出了该基因是桑树种质间耐寒性变异的关键决定因素，其表达与芽萌发时间直接相关。这为在育种计划中基于芽萌发时间选择耐寒桑树种质提供了分子基础。
