Guo Pengyu, Cheng Xin, Wang Yunshu, Chen Guoping, Chen Xuqing, Yang Yingwu, Zhang Xiuhai, Hu Zongli
Laboratory of Molecular Biology of Tomato, Bioengineering College, Chongqing University, Chongqing 400044, China.
Institute of Grassland, Flowers and Ecology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
Int J Mol Sci. 2024 Dec 14;25(24):13419. doi: 10.3390/ijms252413419.
The bHLH (basic Helix-Loop-Helix) transcription factor serves as pivotal controller in plant growth and development. In a previous study, the overexpression of in L. Ailsa Craig (AC) altered the JA (Jasmonic acid) response and endogenous GA (Gibberellic acid) content. However, the detailed regulation mechanism was not fully explored. In the present research, we found that the overexpression of influenced the accumulation of GA, JA and BR (Brassinolide). RNA-Seq data illustrated that the expression levels of genes related to these plant hormones were significantly affected. Additionally, the interaction of SlUPA-like with SlMYB21, SlMYC2 and SlDELLA was characterized by employing Y2H (Yeast Two-Hybrid) and BiFC (Bimolecular Fluorescence Complementation) assay. Furthermore, Dual-LUC (Dual-Luciferase) assay and EMSA (Electrophoretic Mobility Shift Assay) identified that SlUPA-like directly targeted the E-box motif in the promoter of and activated the transcription of . These results shed light on the potential role of SlUPA-like in mediating crosstalk among multiple plant hormones and established a robust theoretical framework for further unraveling the functions of SlUPA-like transcription factors in the context of plant growth and hormone signal transduction.
bHLH（碱性螺旋-环-螺旋）转录因子在植物生长发育过程中起着关键调控作用。在之前的一项研究中，在艾尔莎·克雷格番茄（AC）中过表达[具体基因未给出]改变了茉莉酸（JA）响应和内源赤霉素（GA）含量。然而，详细的调控机制尚未得到充分探究。在本研究中，我们发现过表达[具体基因未给出]影响了GA、JA和油菜素内酯（BR）的积累。RNA测序数据表明，与这些植物激素相关的基因表达水平受到显著影响。此外，通过酵母双杂交（Y2H）和双分子荧光互补（BiFC）实验对类似SlUPA与SlMYB21、SlMYC2和SlDELLA之间的相互作用进行了表征。此外，双荧光素酶（Dual-LUC）实验和电泳迁移率变动分析（EMSA）确定类似SlUPA直接靶向[具体基因未给出]启动子中的E-box基序并激活其转录。这些结果揭示了类似SlUPA在介导多种植物激素相互作用中的潜在作用，并为进一步阐明类似SlUPA转录因子在植物生长和激素信号转导背景下的功能建立了坚实的理论框架。
