Modulates Dopamine to Increase Sugar Responsiveness in Honeybees ().

The appetite of honeybees for food is crucial to their survival and reproduction, as they sustain their entire colony by collecting pollen and nectar for nutrients. Dopamine, an important neurotransmitter, regulates appetite and satiety. However, how dopamine regulates honeybee foraging behavior remains unexplored. In this study, we investigated dopamine expression in 23-day-old under different food-wanting conditions and identified differentially expressed genes (DEGs) in the brains of honeybees using RNA sequencing technology. We showed that dopamine levels in honeybees starved for 2 h were higher than those sated after 2 h of starvation. RNA-seq results revealed there were differences in the expression of cytochrome P450-dependent monooxygenase () in honeybees, which regulated the sucrose sensitivity of honeybees under different intake states. Furthermore, targeted the expression of the to promote dopamine synthesis. Our findings emphasize the relationship between dopamine and honeybees' desire for food at the molecular level, providing a reference for further exploring the mechanism of food wanting.