Drzewiecka Dominika, Levina Evgeniya A, Shashkov Alexander S, Kalinchuk Nadezhda A, Knirel Yuriy A
Department of Biology of Bacteria, Faculty of Biology and Environmental Protection, University of Lodz, 90-237 Lodz, Poland.
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia.
Int J Mol Sci. 2024 Dec 20;25(24):13642. doi: 10.3390/ijms252413642.
Bacteria from the genus are facultative human pathogens, primarily attacking the urinary tract and wounds. A total of 85 O serogroups have been identified so far among these bacilli. Bprz 86 was isolated from the fistula of a patient in Łódź, Poland. Enzyme-Linked Immunosorbent Assay (ELISA) and Western blotting studies involving the Bprz 86 lipopolysaccharide (LPS) and the strain-specific rabbit antiserum indicated that the strain, which does not belong to any of the O1-O85 serogroups, shares a common epitope with O17 antigens and is identical to another clinical strain, Sm 120, isolated from the urine of a patient in the area. The O-specific polysaccharide (O antigen) was obtained from Bprz 86 LPS through mild acid degradation, and the six-constituent structure of its repeating unit was determined using chemical analyses and 1D and 2D H and C Nuclear Magnetic Resonance (NMR) spectroscopy. It includes ()-3-hydroxybutanoyl, which, along with fucosamine and glucose residues, forms a fragment also present in the O17 antigens. Based on the obtained serological and chemical data, the two studied isolates were proposed as candidates for a new successive O serogroup in the genus , O86.
该属细菌是兼性人类病原体，主要侵袭泌尿道和伤口。迄今为止，在这些杆菌中已鉴定出总共85个O血清群。Bprz 86是从波兰罗兹一名患者的瘘管中分离出来的。涉及Bprz 86脂多糖（LPS）和菌株特异性兔抗血清的酶联免疫吸附测定（ELISA）和蛋白质印迹研究表明，该菌株不属于O1 - O85血清群中的任何一个，与O17抗原具有共同表位，并且与从该地区一名患者尿液中分离出的另一临床菌株Sm 120相同。通过温和酸降解从Bprz 86 LPS中获得O特异性多糖（O抗原），并使用化学分析以及一维和二维氢和碳核磁共振（NMR）光谱确定其重复单元的六组分结构。它包括（）-3-羟基丁酰基，其与岩藻糖胺和葡萄糖残基一起形成O17抗原中也存在的一个片段。基于获得的血清学和化学数据，所研究的两种分离株被提议作为该属中一个新的连续O血清群O86的候选者。
