Lecturer at Basic Science Department, College of Medicine, Hawler Medical University, Erbil, Kurdistan Region-Iraq.
Department of Biology, College of Education, Salahaddin University-Erbil, Erbil, Kurdistan Region- Iraq.
Cell Mol Biol (Noisy-le-grand). 2021 Nov 25;67(3):11-23. doi: 10.14715/cmb/2021.67.3.3.
The use of plant extracts represents a promising approach for the synthesis of silver nanoparticles (AgNPs). This study reports the low-cost, green synthesis of AgNPs using the extract of clove and black seeds. The biosynthesized AgNPs were confirmed and characterized by analysis of the spectroscopy profile of the UV-visible spectrophotometer. The purpose of the present study is to evaluate the inhibitory effect concentration (MIC) of AgNPs, clove, and black cumin seed extracts on the growth and swarming of P. mirabilis. Clinical isolates of P. mirabilis were isolated from patients suffering from urinary tract infections. Thirteen types of antibiotics were used in the present study to detect their ability to inhibit P. mirabilis's resistance. Immunological findings included the determination of serum levels of IgG, IgM, IgA and complement protein C3 and C4. Results showed that IgG and IgA concentrations significantly increased (1311.13 ± 72.54 and 279 ± 21.31) respectively in UTI patients in comparison to the healthy control group which was 1089.88 ± 37.33 and 117.611 ± 4.19 respectively, While IgM concentrations were increased non significantly in UTI patients (153.331 ± 6.45) in comparison to healthy control (145.2 ± 13.49). Complement components C3 showed a significant increase in UTI patients with mean values of 125.95 ± 6.22 compared to the control group with mean values of 55.191 ± 9.64, while C4 showed statically non-significant among UTI patients in comparison with the control group (35.195 ± 2.34 and 34.371 ± 1.22) respectively.
使用植物提取物代表了一种有前途的方法，可以合成银纳米粒子（AgNPs）。本研究报告了使用丁香和黑种子提取物低成本、绿色合成 AgNPs 的方法。通过分析紫外可见分光光度计的光谱图，对生物合成的 AgNPs 进行了确认和表征。本研究的目的是评估 AgNPs、丁香和黑孜然种子提取物对奇异变形杆菌生长和群集的抑制浓度（MIC）。奇异变形杆菌的临床分离株从患有尿路感染的患者中分离出来。本研究使用了 13 种抗生素来检测它们抑制奇异变形杆菌耐药性的能力。免疫学发现包括测定 IgG、IgM、IgA 和补体蛋白 C3 和 C4 的血清水平。结果表明，与健康对照组（分别为 1089.88 ± 37.33 和 117.611 ± 4.19）相比，尿路感染患者的 IgG 和 IgA 浓度分别显著升高（分别为 1311.13 ± 72.54 和 279 ± 21.31），而 IgM 浓度在尿路感染患者中升高不显著（153.331 ± 6.45）与健康对照组（145.2 ± 13.49）相比。补体成分 C3 在尿路感染患者中显著增加，平均值为 125.95 ± 6.22，而与健康对照组相比，平均值为 55.191 ± 9.64，而 C4 在尿路感染患者中的变化不显著与健康对照组（分别为 35.195 ± 2.34 和 34.371 ± 1.22）。
