Gotardi Guilherme A, Batista Natália R F, Ishizuka Tamylin Kaori, Marques Luiz H, Dal Pogetto Mário H, Sethi Amit, Dahmer Mark L, Nowatzki Timothy
Corteva Agriscience, Rodovia SP 147 Km 71, Mogi Mirim 13801-540, SP, Brazil.
Corteva Agriscience, 9330 Zionsville, Indianapolis, IN 46268, USA.
Insects. 2024 Dec 4;15(12):969. doi: 10.3390/insects15120969.
and are two species belonging to the Plusiinae subfamily within the Noctuidae family. Due to their morphological similarity, the identification of their larvae is difficult and time-consuming. A rapid and accurate identification of these two species is essential for their management as these species exhibit differential susceptibilities to insecticides and crops engineered to express () proteins, and a molecular tool can easily provide this differentiation. Currently, molecular analysis can identify these species through genetic sequencing, an expensive and time-consuming process. In our study, after sequencing part of the mtDNA cytochrome c oxidase I () gene and based on the differences found in the gene of each species, a set of species-specific primers was developed: one reverse primer common to both species and two forward primers, specific to each species, amplifying fragments of 199 base pairs (bp) for and 299 bp for . Our results indicate that the primers were specific for these species, enabling the identification of individuals directly through agarose gel. The new methodology proved to be accurate, rapid, and reliable for the correct identification of these two species of loopers.
[具体物种名称1]和[具体物种名称2]是夜蛾科Plusinae亚科的两个物种。由于它们在形态上相似，其幼虫的鉴定既困难又耗时。快速准确地鉴定这两个物种对于它们的管理至关重要，因为这些物种对杀虫剂和经基因工程改造以表达[具体蛋白名称]蛋白的作物表现出不同的敏感性，而分子工具可以轻松实现这种区分。目前，分子分析可以通过基因测序来鉴定这些物种，这是一个昂贵且耗时的过程。在我们的研究中，对线粒体DNA细胞色素c氧化酶I（COI）基因的部分进行测序后，基于在每个物种基因中发现的差异，开发了一组物种特异性引物：一个两个物种通用的反向引物和两个分别针对每个物种的正向引物，扩增出的片段对于[具体物种名称1]为199个碱基对（bp），对于[具体物种名称2]为299个碱基对。我们的结果表明，这些引物对这些物种具有特异性，能够直接通过琼脂糖凝胶鉴定个体。新方法被证明对于正确鉴定这两种尺蠖蛾准确、快速且可靠。
