Kornienko Igor V, Aramova Olga Yu, Tishchenko Anna A, Rudoy Dmitriy V, Chikindas Michael Leonidas
Center for Agrobiotechnology, Don State Technical University, Gagarina Sq. 1, Rostov-on-Don 344003, Russia.
Federal Research Centre Southern Scientific Centre of the Russian Academy of Sciences, Chekhov Ave. 41, Rostov-on-Don 344006, Russia.
Molecules. 2024 Dec 18;29(24):5978. doi: 10.3390/molecules29245978.
The stability of RNA is a critical factor in determining its functionality and degradation in the cell. In recent years, it has been shown that the stability of RNA depends on a complex interaction of external and internal factors. External conditions, such as temperature fluctuations, the level of acidity of the environment, the presence of various substances and ions, as well as the effects of oxidative stress, can change the structure of RNA and affect its stability. Internal factors, including the specific structural features of RNA and its interactions with protein molecules, also have a significant impact on the regulation of the stability of these molecules. In this article, we review the main factors influencing RNA stability, since understanding the factors influencing this extremely complex process is important not only for understanding the regulation of expression at the RNA level but also for developing new methods for isolating and stabilizing RNA in preparation for creating biobanks of genetic material. We reviewed a modern solution to this problem and formulated basic recommendations for RNA storage aimed at minimizing degradation and damage to the molecule.
RNA的稳定性是决定其在细胞内功能及降解的关键因素。近年来，研究表明RNA的稳定性取决于外部和内部因素的复杂相互作用。外部条件，如温度波动、环境酸度水平、各种物质和离子的存在以及氧化应激的影响，均可改变RNA的结构并影响其稳定性。内部因素，包括RNA的特定结构特征及其与蛋白质分子的相互作用，对这些分子稳定性的调节也具有重大影响。在本文中，我们综述了影响RNA稳定性的主要因素，因为了解影响这一极其复杂过程的因素不仅对于理解RNA水平的表达调控很重要，而且对于开发用于分离和稳定RNA以创建遗传物质生物样本库的新方法也很重要。我们回顾了针对此问题的现代解决方案，并制定了关于RNA储存的基本建议，旨在最大程度减少分子的降解和损伤。
