Gomulski Jan, Krzemińska Marta, Jochymek Magdalena, Kiss Anna K, Grzegorczyk-Karolak Izabela
Department of Biology and Pharmaceutical Botany, Medical University of Lodz, 90-151 Lodz, Poland.
Department of Pharmaceutical Biology, Medical University of Warsaw, 02-097 Warsaw, Poland.
Molecules. 2024 Dec 19;29(24):5983. doi: 10.3390/molecules29245983.
Plants of the genus, known for their rich phytochemical profiles, are used in traditional Chinese, Korean, Japanese, and Indian medicine to treat various ailments, including inflammation, hypertension, diabetes, hyperlipidemia, and cancer. Due to the limited natural availability of these plants, there is a growing interest in utilizing in vitro culture techniques to produce their bioactive compounds sustainably. In this study, the effects are compared of Murashige and Skoog (MS), Woody Plant medium (WP), Gamborg B5 (B5), and Schenk and Hildebrandt (SH) basal media on growth, biomass accumulation, and polyphenolic compound production in shoot cultures of and . The composition of the culture medium significantly influenced the growth and metabolic profiles of both species. exhibited the highest proliferation potential on WP and SH media, while was similar on WP, SH, and B5 media (multiplication factor of about 20). Dry weight accumulation was highest in grown on SH medium (0.292 g/culture), while achieved a comparable biomass on SH and WP media (0.240 g/culture and 0.228 g/culture, respectively). The chemical analysis showed similar secondary metabolite profiles between the two species with phenylethanoids such as acteoside being the predominant bioactive compounds in hydromethanolic extracts. WP medium was the most favorable for polyphenol accumulation in (64.5 mg/g DW), while the SH medium yielded the highest total polyphenol content in (36.6 mg/g DW). In this study, the importance is underscored of basal medium selection in optimizing the in vitro production of bioactive polyphenolic compounds in species, providing a foundation for the sustainable and scalable production of these pharmacologically significant metabolites.
该属植物以其丰富的植物化学特征而闻名，在中国、韩国、日本和印度传统医学中用于治疗各种疾病，包括炎症、高血压、糖尿病、高脂血症和癌症。由于这些植物的天然可获得量有限，人们越来越有兴趣利用体外培养技术可持续地生产其生物活性化合物。在本研究中，比较了Murashige和Skoog（MS）培养基、木本植物培养基（WP）、Gamborg B5（B5）培养基和Schenk和Hildebrandt（SH）基本培养基对[植物名称1]和[植物名称2]茎段培养物生长、生物量积累和多酚类化合物产生的影响。培养基的组成显著影响了两个物种的生长和代谢特征。[植物名称1]在WP和SH培养基上表现出最高的增殖潜力，而[植物名称2]在WP、SH和B5培养基上表现相似（增殖系数约为20）。在SH培养基上生长的[植物名称1]干重积累最高（0.292 g/培养物），而[植物名称2]在SH和WP培养基上获得了相当的生物量（分别为0.240 g/培养物和0.228 g/培养物）。化学分析表明，两个物种的次生代谢产物谱相似，苯乙醇苷类如毛蕊花糖苷是甲醇提取物中的主要生物活性化合物。WP培养基最有利于[植物名称1]中多酚的积累（64.5 mg/g干重），而SH培养基在[植物名称2]中产生的总多酚含量最高（36.6 mg/g干重）。在本研究中，强调了基础培养基选择在优化[植物名称]物种生物活性多酚化合物体外生产中的重要性，为这些具有药理学意义的代谢产物的可持续和可扩展生产提供了基础。
