Department of Biology and Pharmaceutical Botany, Medical University of Lodz, Muszynskiego 1, 90-151 Lodz, Poland.
Department of Pharmacognosy and Molecular Basis of Phytotherapy, Warsaw Medical University, Banacha 1, Warsaw 02-097, Poland.
Biomolecules. 2023 Jan 24;13(2):227. doi: 10.3390/biom13020227.
is a plant native to the Chinese Yunnan Province. This species has been used in traditional Chinese medicine as a substitute for Danshen (the roots of ). The aim of our study was to establish an effective system for propagating shoots to obtain large amounts of material rich in bioactive compounds. Phytohormones were used to regulate shoot growth and regeneration potential and influence plant secondary metabolism. The shoot tips were incubated on a Murashige and Skoog agar medium supplemented with 0.1 or 0.5 mg/L IAA (indole-3-acetic acid) and the cytokinins benzylaminopurine (BAP), meta-topoline (M-T), 6-benzylaminopurine riboside (RBAP), N-benzyl-9-(2-tetrahydropyranyl)-adenine (BPA) or kinetin, (K) at concentrations of 0.5, 1 or 2 mg/L. It was observed that the type and concentration of growth regulator significantly influenced the regeneration potential of shoots. The highest multiplication rate was obtained when 0.1 mg/L IAA and 2 mg/L BPA were used. Under these conditions, 100% of shoot tips formed buds and almost seven buds/shoot per explant were obtained after five weeks. Meanwhile, the highest biomass was found for shoots growing on a medium supplemented with 0.1 mg/L IAA and 1 mg/L M-T: 1.2 g of fresh weight and 0.17 g of dry weight. However, a medium with 0.1 mg/L IAA and 2 mg/L RBAP was most favorable for bioactive phenolic acid content, with a total polyphenol level (37.7 mg/g dw) 4.5 times higher than in shoots grown on medium without growth regulators (8.23 mg/g dw). Finally, optimal conditions were selected by TOPSIS (technique for order of preference by similarity to the ideal solution); the culture of grown on an MS medium containing 0.1 mg/L IAA and 1 mg/L M-T was found to be the most efficient for polyphenol accumulation and can be used for the production of medicinally relevant compounds.
是一种原产于中国云南省的植物。该物种已在中国传统医学中被用作丹参（根）的替代品。我们的研究目的是建立一种有效的系统来繁殖 茎，以获得大量富含生物活性化合物的材料。植物激素被用来调节茎的生长和再生潜力，并影响植物的次生代谢。将茎尖接种在添加 0.1 或 0.5 mg/L 吲哚-3-乙酸（IAA）和细胞分裂素苄氨基嘌呤（BAP）、meta-托泊啉（M-T）、6-苄氨基嘌呤核苷（RBAP）、N-苄基-9-（2-四氢吡喃基）-腺嘌呤（BPA）或激动素（K）的 Murashige 和 Skoog 琼脂培养基上，浓度分别为 0.5、1 或 2 mg/L。结果表明，生长调节剂的类型和浓度显著影响 茎的再生潜力。当使用 0.1 mg/L IAA 和 2 mg/L BPA 时，获得了最高的增殖率。在这些条件下，100%的茎尖形成芽，每个外植体获得近 7 个芽/茎，5 周后。同时，在添加 0.1 mg/L IAA 和 1 mg/L M-T 的培养基上生长的茎获得了最高的生物量：新鲜重 1.2 g，干重 0.17 g。然而，含有 0.1 mg/L IAA 和 2 mg/L RBAP 的培养基最有利于生物活性酚酸含量，总多酚水平（37.7 mg/g dw）比不含生长调节剂的培养基（8.23 mg/g dw）高 4.5 倍。最后，通过 TOPSIS（逼近理想解的排序技术）选择最佳条件；发现含有 0.1 mg/L IAA 和 1 mg/L M-T 的 MS 培养基中生长的 最有利于多酚积累，可用于生产药用相关化合物。
