Pardo de Donlebún Blanca, Chabni Assamae, Bañares Celia, Torres Carlos F
Department of Bioactivity and Food Analysis, Institute of Food Science Research (CIAL, CSIC-UAM), C/Nicolas Cabrera 9, Cantoblanco Campus, Autonomous University of Madrid, 28049 Madrid, Spain.
Department of Production and Characterization of Novel Foods, Institute of Food Science Research (CIAL, CSIC-UAM), C/Nicolas Cabrera 9, Cantoblanco Campus, Autonomous University of Madrid, 28049 Madrid, Spain.
Molecules. 2024 Dec 21;29(24):6032. doi: 10.3390/molecules29246032.
It is well stablished that docosahexaenoic (DHA) and arachidonic (ARA) acids fulfill relevant biological activities, especially in newborns. However, oils containing these fatty acids are not always optimally digestible. To address this, various formulation strategies and lipid delivery systems have been developed. This study compares the following three formulations in an digestion model to assess bioaccessibility: Enfamil DHA & ARA (Mead Johnson & Company), an emulsion of Formulaid, AquaCelle, and pasteurized donated human milk, and a previously characterized enzymatic glycerolysis product (GP) of ARA oil and microalgae oil in a 2:1 (:) ratio. To evaluate digestibility, parameters such as the percentage of oily phase (OP), micellar phase (MP), free fatty acids, and monoacylglycerols in the digestion product (DP) were considered. Additionally, diacylglycerol content in the MP can be used as an indirect marker of the emulsification capacity of the DP, and consequently, as an indicator of bioaccessibility. The GP demonstrated the highest bioaccessibility, with a DP containing more than 80% MP (<14% OP), rich in free fatty acids (60%) and monoacylglycerols (17%). Furthermore, more than 40% of total diacylglycerols were present in MP, highlighting GPs' potential as a superior delivery system for DHA and ARA in preterm infant formulations.
已充分证实，二十二碳六烯酸（DHA）和花生四烯酸（ARA）具有相关生物活性，尤其是在新生儿中。然而，含有这些脂肪酸的油脂并不总是具有最佳消化率。为了解决这一问题，人们开发了各种配方策略和脂质递送系统。本研究在消化模型中比较了以下三种配方，以评估生物可及性：美赞臣DHA&ARA（美赞臣公司）、一种由Formulaid、AquaCelle和巴氏杀菌捐赠人乳组成的乳液，以及一种先前表征的ARA油和微藻油以2:1（:）比例的酶促甘油解产物（GP）。为了评估消化率，考虑了消化产物（DP）中油相（OP）、胶束相（MP）、游离脂肪酸和单酰甘油的百分比等参数。此外，MP中的二酰甘油含量可用作DP乳化能力的间接指标，因此也可作为生物可及性的指标。GP表现出最高的生物可及性，其DP含有超过80%的MP（<14%的OP），富含游离脂肪酸（60%）和单酰甘油（17%）。此外，MP中存在超过40%的总二酰甘油，突出了GP作为早产儿配方中DHA和ARA的优质递送系统的潜力。
