Enyetornye Ben, Abugri Henry A, Kusi-Appiah Ama K, Maboni Grazieli, Odoom Theophilus, Gottdenker Nicole L, Velayudhan Binu T
Athens Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA.
Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA.
Pathogens. 2024 Dec 17;13(12):1115. doi: 10.3390/pathogens13121115.
Infectious bursal disease (IBD) continues to threaten poultry production globally, with highly virulent strains circulating in many parts of Africa. In this study, molecular characterization was performed on a circulating infectious bursal disease virus (IBDV) strain from an outbreak in a layer flock in Ghana. Layer chicks presented for necropsy had markedly enlarged and hemorrhagic bursae of Fabricius, with necrotic foci and catarrhal exudate on the serosal surface. Histopathology of the bursa of Fabricius revealed scattered to effacing hemorrhages on the plicae, extensive necrosis with expansion of the stroma between the follicles, and depletion of lymphocytes within the interfollicular epithelium. Reverse transcription polymerase chain reaction (RT-PCR) and subsequent sequencing of the VP2 gene showed the presence of IBDV in formalin-fixed paraffin-embedded tissues. A phylogenetic analysis compared 62 other IBDV sequences from different parts of the world and placed the Ghanaian IBDV in genogroup 3 (vvIBDV), closely related to IBDV from Nigeria. In comparison to reference vvIBDV, there were amino acid substitutions at positions 252, 254, and 300. To the best of our knowledge, this is the first report in which an IBDV from a disease outbreak in Ghana has been sequenced and compared with other IBDVs in a phylogenetic analysis.
传染性法氏囊病（IBD）继续在全球范围内威胁着家禽生产，非洲许多地区都有高致病性毒株在传播。在本研究中，对加纳一个蛋鸡群疫情中流行的传染性法氏囊病病毒（IBDV）毒株进行了分子特征分析。送检尸检的蛋鸡雏鸡法氏囊明显肿大且出血，浆膜表面有坏死灶和卡他性渗出物。法氏囊的组织病理学检查显示皱襞上有散在至融合性出血、广泛坏死伴卵泡间基质扩张以及滤泡间上皮内淋巴细胞减少。逆转录聚合酶链反应（RT-PCR）及随后的VP2基因测序显示在福尔马林固定石蜡包埋组织中存在IBDV。系统发育分析比较了来自世界不同地区的62个其他IBDV序列，将加纳IBDV置于基因群3（vvIBDV）中，与来自尼日利亚的IBDV密切相关。与参考vvIBDV相比，在第252、254和300位氨基酸处有替换。据我们所知，这是首次对加纳疾病暴发中的IBDV进行测序并在系统发育分析中与其他IBDV进行比较的报告。
