Xue Shi-Yuan, Ma Wei, Li Meng-Yuan, Meng Wei-Kang, Ding Yu-Lin, Yang Bo, Lv Yue-Rong, Chen Rui-Bin, Wu Zhi-Hong, Tunala Siqin, Zhang Rong, Zhao Li, Liu Yong-Hong
College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010010, China.
Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China.
Pathogens. 2024 Dec 18;13(12):1118. doi: 10.3390/pathogens13121118.
Paratuberculosis (PTB), primarily caused by subsp. (MAP), is a chronic infection that affects ruminants and is difficult to prevent, diagnose, and treat. Investigating how MAP infections affect the gut microbiota in sheep can aid in the prevention and treatment of ovine PTB. This study examined fecal samples from eight small-tail Han sheep (STHS) at various stages of infection and from three different field areas. All samples underwent DNA extraction and 16S rRNA sequencing. Among all samples, the phyla p. Firmicutes and p. Bacteroidota exhibited the highest relative abundance. The dominant genera in groups M1-M6 were UCG-005, _R-7_group, _RC9_gut_group, , UCG-005, and , whereas those in groups A-C were _R-7_group, , and , respectively. The microbial community structure varied significantly among groups M1-M6. Specifically, 56 microbiota consortia with different taxonomic levels, including the order Clostridiales, were significantly enriched in groups M1-M6, whereas 96 microbiota consortia at different taxonomic levels, including the family , were significantly enriched in groups A-C. To the best of our knowledge, this is the first study to report that MAP infection alters the intestinal microbiota of STHS. Changes in p. Firmicutes abundance can serve as a potential biomarker to distinguish MAP infection and determine the infection stage for its early diagnosis. Our study provides a theoretical basis for the treatment of PTB by regulating the intestinal microbiota, including p. Firmicutes.
副结核病（PTB）主要由副结核分枝杆菌亚种（MAP）引起，是一种影响反刍动物的慢性感染，难以预防、诊断和治疗。研究MAP感染如何影响绵羊的肠道微生物群有助于预防和治疗绵羊PTB。本研究检测了来自八个处于不同感染阶段的小尾寒羊（STHS）以及三个不同牧区的粪便样本。所有样本均进行了DNA提取和16S rRNA测序。在所有样本中，厚壁菌门和拟杆菌门的相对丰度最高。M1 - M6组中的优势属为UCG - 005、_R - 7_组、_RC9_肠道组、UCG - 005，而A - C组中的优势属分别为_R - 7_组、 、 。M1 - M6组之间的微生物群落结构差异显著。具体而言，包括梭菌目在内的56个不同分类水平的微生物群落联合体在M1 - M6组中显著富集，而包括 科在内的96个不同分类水平的微生物群落联合体在A - C组中显著富集。据我们所知，这是第一项报道MAP感染会改变STHS肠道微生物群的研究。厚壁菌门丰度的变化可作为区分MAP感染和确定感染阶段以进行早期诊断的潜在生物标志物。我们的研究为通过调节包括厚壁菌门在内的肠道微生物群来治疗PTB提供了理论依据。
