Binsuwaidan Reem, El-Masry Thanaa A, El-Nagar Maysa M F, El Zahaby Enas I, Gaballa Mohamed M S, El-Bouseary Maisra M
Department of Pharmaceutical Sciences, College of Pharmacy, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia.
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tanta University, Tanta 31527, Egypt.
Pharmaceuticals (Basel). 2024 Nov 27;17(12):1600. doi: 10.3390/ph17121600.
The potent antioxidant lycopene has attracted a large amount of research attention given its potential health benefits. We aimed to assess the antimicrobial, anti-inflammatory, and antioxidant properties of lycopene (Lyc), selenium nanoparticles (Se-NPs), and lycopene selenium nanoparticles (Lyc-Se-NPs).
FTIR, polydispersity index, and zeta potential evaluations provided a complete characterization of the synthesized Lyc-Se-NPs. The broth dilution method and a crystal violet microtiter plate assay were employed to assess the antibacterial and antibiofilm activity, respectively. The rat wound infection model was performed to study the anti-inflammatory effect.
The Lyc-Se-NPs had a zeta potential range of -16.93 to -31.04 mV and a mean particle size of 126.6 ± 3.12 nm. All peaks' percentage transmittance decreased, according to the FTIR analysis of the Lyc-Se-NPs, with the exception of one peak at 2924.22 cm, which is suggestive of C-H stretching. The mean scavenging concentrations for Lyc-Se-NPs in the DPPH and ABTS radical scavenging experiments were 3.85 ± 0.65 and 4.26 ± 0.7 µg/mL, respectively. For , the Lyc-Se-NPs' MIC values varied from 64 to 1024 µg/mL. CLSM verified that treated with sub-MICs of Lyc-Se-NPs showed a significant reduction in biofilm formation. Furthermore, the group treated with 50 mg of Lyc-Se-NPs showed the quickest rate of wound healing. They demonstrated a notable elevation of the HO content in skin tissues, together with the greatest downregulation of TNF-α, IL-1β, and COX-2.
The distinguishing features of Lyc-Se-NPs reveal that this unique compound is a promising antibacterial, antioxidant, and anti-inflammatory agent.
强效抗氧化剂番茄红素因其潜在的健康益处而备受大量研究关注。我们旨在评估番茄红素（Lyc）、硒纳米颗粒（Se-NPs）和番茄红素硒纳米颗粒（Lyc-Se-NPs）的抗菌、抗炎和抗氧化特性。
傅里叶变换红外光谱（FTIR）、多分散指数和zeta电位评估对合成的Lyc-Se-NPs进行了全面表征。采用肉汤稀释法和结晶紫微量滴定板法分别评估抗菌和抗生物膜活性。通过大鼠伤口感染模型研究其抗炎作用。
Lyc-Se-NPs的zeta电位范围为-16.93至-31.04 mV，平均粒径为126.6±3.12 nm。根据Lyc-Se-NPs的FTIR分析，除了在2924.22 cm处的一个峰表明C-H伸缩振动外，所有峰的百分透过率均降低。在二苯基苦味酰基自由基（DPPH）和2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）二铵盐（ABTS）自由基清除实验中，Lyc-Se-NPs的平均清除浓度分别为3.85±0.65和4.26±0.7 μg/mL。对于……，Lyc-Se-NPs的最低抑菌浓度（MIC）值在64至1024 μg/mL之间。共聚焦激光扫描显微镜（CLSM）证实，用低于MIC的Lyc-Se-NPs处理后生物膜形成显著减少。此外，用50 mg Lyc-Se-NPs处理的组伤口愈合速度最快。它们显示皮肤组织中血红素加氧酶（HO）含量显著升高，同时肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和环氧化酶-2（COX-2）的下调幅度最大。
Lyc-Se-NPs的显著特性表明，这种独特的化合物是一种有前景的抗菌、抗氧化和抗炎剂。
