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鸡肉和内脏中阿尔伯特埃希氏菌的检测。

Detection of Escherichia albertii from chicken meat and giblets.

作者信息

Maeda Eriko, Murakami Koichi, Sera Nobuyuki, Ito Kenitiro, Fujimoto Shuji

机构信息

Fukuoka Institute of Health and Environmental Sciences, 39 Mukaizano, Dazaifu, Fukuoka 818-0135, Japan.

出版信息

J Vet Med Sci. 2015 Jul;77(7):871-3. doi: 10.1292/jvms.14-0640. Epub 2015 Mar 8.

DOI:10.1292/jvms.14-0640
PMID:25754935
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4527513/
Abstract

Escherichia albertii occasionally causes food-borne outbreaks of gastroenteritis in humans; however, little is known about the vehicle of transmission. To screen retail chicken products for the presence of E. albertii, 104 retail chicken products were investigated. Portions of enrichment cultures that were PCR-positive for E. albertii (n=3) were sub-cultured on agar medium. Only 2 strains obtained from 2 chicken giblet samples were identified as E. albertii by multi locus sequence typing. Antimicrobial susceptibility testing showed that 1 strain was resistant to streptomycin and sulfisoxazole. Both strains harbored the virulence genes cdt and eae. This study is the first description of E. albertii isolation from retail food, suggesting that chicken products are a potential vehicle of E. albertii transmission.

摘要

艾氏大肠杆菌偶尔会引起人类食源性肠胃炎暴发;然而,关于其传播媒介知之甚少。为了筛查零售鸡肉产品中是否存在艾氏大肠杆菌,对104份零售鸡肉产品进行了调查。对艾氏大肠杆菌PCR呈阳性的富集培养物部分(n = 3)在琼脂培养基上进行传代培养。通过多位点序列分型,仅从2份鸡内脏样品中获得的2株菌株被鉴定为艾氏大肠杆菌。药敏试验表明,1株对链霉素和磺胺异恶唑耐药。两株菌株均携带毒力基因cdt和eae。本研究首次描述了从零售食品中分离出艾氏大肠杆菌,表明鸡肉产品是艾氏大肠杆菌传播的潜在媒介。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/157d/4527513/e789dbd8cd75/jvms-77-871-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/157d/4527513/e789dbd8cd75/jvms-77-871-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/157d/4527513/e789dbd8cd75/jvms-77-871-g001.jpg

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