Hassan Jayedul, Utsho Kishor Sosmith, Karmakar Susmita, Ali Md Wohab, Awasthi Sharda Prasad, Uyama Chiharu, Hatanaka Noritoshi, Yamasaki Shinji, Hinenoya Atsushi
Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.
Graduate School of Veterinary Science, Osaka Metropolitan University, Osaka, Japan; Asian Health Science Institute, Osaka Metropolitan University, Osaka, Japan; Osaka International Research Center for Infectious Diseases, Osaka Metropolitan University, Osaka, Japan.
Int J Food Microbiol. 2025 Mar 2;431:111081. doi: 10.1016/j.ijfoodmicro.2025.111081. Epub 2025 Jan 22.
Escherichia albertii is an emerging zoonotic pathogen linked to human gastrointestinal illnesses, with poultry meats being considered as a key source of human infections. However, there is little information regarding the distribution and characteristics of this bacterium in Bangladesh. This study investigated the occurrence, antimicrobial resistance, and virulence of E. albertii in chicken meats from retail outlets in Bangladesh. We collected samples from 61 dressed chickens across 17 retail shops from 4 upazilas, along with swabs from cloaca, processing utensils, and worker hands. Detection of E. albertii by species-specific PCR revealed substantial occurrence of E. albertii in retail chicken meat (63.9 %), cloaca (71.4 %), human hand (45.5 %), bleeding cone (13.3 %) and blade (10 %). Almost all the E. albertii isolates (94.4 %) exhibited resistance to at least one of the tested antimicrobials, among which 50 % were multidrug resistant, including resistance to clinically relevant antimicrobials such as tetracycline, ampicillin, gentamicin, kanamycin, nalidixic acid and ciprofloxacin. Whole genome sequencing analysis identified the presence of corresponding antimicrobial resistance genes and critical virulence genes (eae, Eacdt). Notably, although wgSNP-based phylogenetic analysis showed the genomic diversity of the isolates, some of the isolates from the same shop displayed clonal relationships among meats, cloacal swabs, and human hand swabs, indicating contamination during processing. These findings highlight the public health risk posed by E. albertii in retail poultry, underlining the poultry's role as a potential vector for zoonotic transmission and the need for improved biosecurity and antimicrobial management practices in poultry production.
阿尔伯蒂埃希氏菌是一种新出现的人畜共患病原体，与人类胃肠道疾病有关，禽肉被认为是人类感染的主要来源。然而，关于这种细菌在孟加拉国的分布和特征的信息很少。本研究调查了孟加拉国零售市场鸡肉中阿尔伯蒂埃希氏菌的存在情况、抗菌药物耐药性和毒力。我们从4个乡的17家零售店的61只屠鸡中采集了样本，以及泄殖腔、加工器具和工人手部的拭子。通过种特异性PCR检测阿尔伯蒂埃希氏菌发现，零售鸡肉（63.9%）、泄殖腔（71.4%）、人手（45.5%）、放血锥（13.3%）和刀片（10%）中大量存在阿尔伯蒂埃希氏菌。几乎所有的阿尔伯蒂埃希氏菌分离株（94.4%）对至少一种测试抗菌药物表现出耐药性，其中50%为多重耐药，包括对四环素、氨苄西林、庆大霉素、卡那霉素、萘啶酸和环丙沙星等临床相关抗菌药物的耐药性。全基因组测序分析确定了相应的抗菌药物耐药基因和关键毒力基因（eae、Eacdt）的存在。值得注意的是，尽管基于wgSNP的系统发育分析显示了分离株的基因组多样性，但来自同一家店的一些分离株在鸡肉、泄殖腔拭子和人手拭子之间显示出克隆关系，表明在加工过程中受到污染。这些发现突出了零售家禽中阿尔伯蒂埃希氏菌对公共卫生的风险，强调了家禽作为人畜共患病传播潜在载体的作用，以及在家禽生产中改善生物安全和抗菌药物管理措施的必要性。
