Stanilov Iskren, Blazhev Alexander, Miteva Lyuba
Department of Molecular Biology, Immunology and Medical Genetics, Faculty of Medicine, Trakia University, 6000 Stara Zagora, Bulgaria.
Department of Biology, Medical University-Pleven, 5800 Pleven, Bulgaria.
Microorganisms. 2023 Feb 26;11(3):594. doi: 10.3390/microorganisms11030594.
The aim of the study was to determine prevalence of -infected ticks in the Black Sea Coast and the Pleven regions of Bulgaria. A total of 350 ticks from different tick species were collected. Two hundred fifty-five ticks were removed from dogs in the Black Sea Coast region, and 95 ticks were collected by flagging vegetation with a white flannel cloth in two areas in the region of Pleven. After the DNA isolation of the ticks, a genus-specific polymerase chain reaction (PCR) was performed to identify Second PCRs were performed with species-specific primers to identify () and (). The results showed that 26.9% of the ticks were infected with in the Black Sea Coast region and 36.8% in the Pleven region. The infection with was detected in 35.7% and in 25.0% of positive ticks from the Black Sea Coast region. In the Pleven region, 22.9% of ticks were positive for , while 42.9% were positive for . The molecular identification of in ticks collected from Bulgaria was performed for the first time. In conclusion, the present study revealed a higher prevalence of ticks infected with , particularly , in the Pleven region than in the Black Sea Coast region.
本研究的目的是确定保加利亚黑海沿岸和普列文地区感染[具体病原体名称未给出]的蜱虫的流行率。共收集了350只来自不同蜱种的蜱虫。其中255只蜱虫是从黑海沿岸地区的狗身上采集的，95只蜱虫是在普列文地区的两个区域用白色法兰绒布对植被进行拖旗法采集的。蜱虫经DNA提取后，进行属特异性聚合酶链反应（PCR）以鉴定[具体病原体名称未给出]。再用种特异性引物进行第二轮PCR以鉴定[具体病原体名称未给出]和[具体病原体名称未给出]。结果显示，黑海沿岸地区26.9%的蜱虫感染了[具体病原体名称未给出]，普列文地区为36.8%。在黑海沿岸地区，35.7%的阳性蜱虫检测到感染了[具体病原体名称未给出]，25.0%检测到感染了[具体病原体名称未给出]。在普列文地区，22.9%的蜱虫[具体病原体名称未给出]呈阳性，42.9%的蜱虫[具体病原体名称未给出]呈阳性。首次对从保加利亚采集的蜱虫进行了[具体病原体名称未给出]的分子鉴定。总之，本研究表明，普列文地区感染[具体病原体名称未给出]，尤其是[具体病原体名称未给出]的蜱虫流行率高于黑海沿岸地区。
