First Identification of Emerging Pathogenic Yeast in (Diptera: Psychodidae) at a Brazilian Hospital.

Psychodinae (Diptera: Psychodidae), commonly known as "drain flies", are a subfamily of insects adapted to environments modified by humans. While often regarded as harmless, it has been reported that they may carry pathogenic microorganisms, contributing to hospital environmental contamination and potentially playing a role in healthcare-associated infections. This study aimed to investigate drain flies in a hospital setting to assess their role in carrying microbial pathogens. Twenty-six drain flies were collected from a tertiary hospital using sterile tubes and processed within two hours. The insects were identified as (Williston, 1893). Whole-body macerates were cultured on EMB media, and fungal isolates were identified using MALDI-TOF MS and ITS region sequencing. The emergent pathogen was isolated, highlighting the potential role of as a mechanical vector of fungal pathogens associated with HAIs. These findings underscore the importance of monitoring drain flies as part of infection prevention and control strategies in healthcare settings.