Vieira-Alcântara Kéren, Chagas Thiago Pavoni Gomes, da Costa Gisela Lara, Pinto Tatiane Nobre, Oliveira Manoel Marques Evangelista, Zahner Viviane
Laboratório de Simulídeos e Oncocercose & Entomologia Médica e Forense, Oswaldo Cruz Instituto, Fiocruz, Avenida Brasil 4365, Manguinhos, Rio de Janeiro 21040-360, Brazil.
Departamento de Patologia, Faculdade de Medicina, Universidade Federal Fluminense, Niterói 24220-008, Brazil.
Microorganisms. 2024 Dec 7;12(12):2520. doi: 10.3390/microorganisms12122520.
Psychodinae (Diptera: Psychodidae), commonly known as "drain flies", are a subfamily of insects adapted to environments modified by humans. While often regarded as harmless, it has been reported that they may carry pathogenic microorganisms, contributing to hospital environmental contamination and potentially playing a role in healthcare-associated infections. This study aimed to investigate drain flies in a hospital setting to assess their role in carrying microbial pathogens. Twenty-six drain flies were collected from a tertiary hospital using sterile tubes and processed within two hours. The insects were identified as (Williston, 1893). Whole-body macerates were cultured on EMB media, and fungal isolates were identified using MALDI-TOF MS and ITS region sequencing. The emergent pathogen was isolated, highlighting the potential role of as a mechanical vector of fungal pathogens associated with HAIs. These findings underscore the importance of monitoring drain flies as part of infection prevention and control strategies in healthcare settings.
毛蠓亚科（双翅目：毛蠓科），通常被称为“下水道蝇”，是一类适应人类改造环境的昆虫亚科。虽然它们通常被认为是无害的，但据报道，它们可能携带致病微生物，导致医院环境污染，并可能在医疗相关感染中发挥作用。本研究旨在调查医院环境中的下水道蝇，以评估它们在携带微生物病原体方面的作用。使用无菌试管从一家三级医院收集了26只下水道蝇，并在两小时内进行处理。这些昆虫被鉴定为（威利斯顿，1893年）。将全身匀浆接种在伊红美蓝培养基上培养，使用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）和内转录间隔区（ITS）测序对真菌分离株进行鉴定。分离出了新兴病原体，突出了下水道蝇作为与医疗相关感染相关真菌病原体的机械传播媒介的潜在作用。这些发现强调了在医疗机构感染预防和控制策略中监测下水道蝇的重要性。
