肠道微生物群参与了暴露于慢性身体应激和高脂饮食的青春期BALB/c小鼠认知灵活性的调节。

The Gut Microbiota Is Involved in the Regulation of Cognitive Flexibility in Adolescent BALB/c Mice Exposed to Chronic Physical Stress and a High-Fat Diet.

作者信息

González Cristian Yuriana, Estrada José Antonio, Oros-Pantoja Rigoberto, Colín-Ferreyra María Del Carmen, Benitez-Arciniega Alejandra Donaji, Soto Piña Alexandra Estela, Aguirre-Garrido José Félix

机构信息

School of Medicine, Autonomous University of the State of Mexico, Paseo Tollocan and Jesús Carranza, Toluca de Lerdo 50180, State of Mexico, Mexico.

Department of Biotechnology and Environmental Microbiology, Autonomous Metropolitan University-Lerma, Hidalgo Pte. 46, Lerma 52006, State of Mexico, Mexico.

出版信息

Microorganisms. 2024 Dec 10;12(12):2542. doi: 10.3390/microorganisms12122542.

DOI:10.3390/microorganisms12122542

PMID:39770745

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11677384/

Abstract

UNLABELLED

Dysfunction in the prefrontal cortex can lead to cognitive inflexibility due to multifactorial causes as included cardiometabolic disorders, stress, inadequate diets, as well as an imbalance of the gut-brain axis microbiota. However, these risk factors have not been evaluated jointly. The purpose of this study was to evaluate the effect of physical stress (MS: Male Stress and FS: Female Stress) and high-fat diet (MD: Male Diet and FD: Female Diet) supplementation on the gut microbiota and cognitive flexibility.

METHODS

The study was performed on 47 mice, 30 male (M) and 17 female (F) BALBc, exposed to chronic stress physical (S) and high-fat diet (D). Cognitive flexibility was evaluated using the Attentional Set-Shifting Test (ASST) and the gut microbiota composition in terms of relative abundance (%) and alpha-beta diversity.

RESULTS

Results showed that S and D reduced cognitive flexibility in male and female mice ( < 0.0001). Significant changes occurred in spp. (MM vs. MS:MD; < 0.0001), spp. (FC vs. FS; = 0.0002; FC vs. FD, = 0.0033); spp. (MC vs. MD, = 0.0008; MM vs. MD, < 0.0001) and spp. (MC vs. MD and FM vs. FS, < 0.0001; FM vs. MD, = 0.0393) genera among groups. Predictive functional analysis (QIIME2 and PICRUSt2) showed a significant increase in the expression of histidine kinase, alanine dehydrogenase, glutamine synthase, glutamate synthase, arginine succinyl synthase, and tryptophan synthase genes ( < 0.05), the latter being a precursor of serotonin (5-HT).

CONCLUSIONS

Chronic physical stress and a high-fat diet modify cognitive flexibility and the composition and predictive function of the gut microbiota.

摘要

未标记

前额叶皮质功能障碍可由于多种因素导致认知灵活性下降，这些因素包括心脏代谢紊乱、压力、饮食不均衡以及肠脑轴微生物群失衡。然而，这些风险因素尚未被联合评估。本研究的目的是评估身体应激（雄性应激：MS和雌性应激：FS）和高脂饮食（雄性饮食：MD和雌性饮食：FD）补充对肠道微生物群和认知灵活性的影响。

方法

本研究对47只小鼠进行，其中30只雄性（M）和17只雌性（F）BALBc小鼠，使其暴露于慢性身体应激（S）和高脂饮食（D）。使用注意力转换测试（ASST）评估认知灵活性，并根据相对丰度（%）和α-β多样性评估肠道微生物群组成。

结果

结果显示，S和D降低了雄性和雌性小鼠的认知灵活性（<0.0001）。在属中发生了显著变化（MM与MS:MD相比；<0.0001），属（FC与FS相比；=0.0002；FC与FD相比，=0.0033）；属（MC与MD相比，=0.0008；MM与MD相比，<0.0001）以及属（MC与MD相比和FM与FS相比，<0.0001；FM与MD相比，=0.0393）。预测功能分析（QIIME2和PICRUSt2）显示组间组氨酸激酶、丙氨酸脱氢酶、谷氨酰胺合成酶、谷氨酸合成酶、精氨酸琥珀酰合成酶和色氨酸合成酶基因的表达显著增加（<0.05），后者是血清素（5-HT）的前体。