Fabersani Emanuel, Marquez Antonela, Russo Matías, Ross Romina, Torres Sebastián, Fontana Cecilia, Puglisi Edoardo, Medina Roxana, Gauffin-Cano Paola
Facultad de Agronomía y Zootecnia, Universidad Nacional de Tucumán, Tucumán, Argentina.
Centro de Referencia para Lactobacilos -CONICET, Tucumán, Argentina.
Front Nutr. 2021 Sep 9;8:718564. doi: 10.3389/fnut.2021.718564. eCollection 2021.
Dietary strategies, including the use of probiotics as preventive agents that modulate the gut microbiota and regulate the function of adipose tissue, are suitable tools for the prevention or amelioration of obesity and its comorbidities. We aimed to evaluate the effect of lactic acid bacteria (LAB) with different adipo- and immuno-modulatory capacities on metabolic and immunological parameters and intestinal composition microbiota in high-fat-diet-induced in mice fed a high-fat diet Balb/c weaning male mice were fed a standard (SD) or high-fat diet (HFD) with or without supplementation with CRL1446 (CRL1446) CRL1434 (CRL1434), or CRL431 (CRL431) for 45 days. Biochemical and immunological parameters, white-adipose tissue histology, gut microbiota composition, and cellular functionality (adipocytes and macrophages) were evaluated in SD and HFD mice. CRL1446 and CRL1434 administration, unlike CRL431, induced significant changes in the body and adipose tissue weights and the size of adipocytes. Also, these strains caused a decrease in plasmatic glucose, cholesterol, triglycerides, leptin, TNF-α, IL-6 levels, and an increase of IL-10. The CRL1446 and CRL1434 obese adipocyte in functionality assays showed, after LPS stimulus, a reduction in leptin secretion compared to obese control, while with CRL431, no change was observed. In macrophages from obese mice fed with CRL1446 and CRL1434, after LPS stimulus, lower levels of MCP-1, TNF-α, IL-6 compared to obese control were observed. In contrast, CRL431 did not induce modification of cytokine values. Regarding gut microbiota, all strain administration caused a decrease in Firmicutes/Bacteroidetes index and diversity. As well as, related to genus results, all strains increased, mainly the genera , and XIVa. CRL1446 induced a higher increase in the genus during the study period. The tested probiotic strains differentially modulated the intestinal microbiota and metabolic/immunological parameters in high-fat-diet-induced obese mice. These results suggest that CRL1446 and CRL1434 strains could be used as adjuvant probiotics strains for nutritional treatment to obesity and overweight. At the same time, the CRL431 strain could be more beneficial in pathologies that require regulation of the immune system.
饮食策略，包括使用益生菌作为调节肠道微生物群和调节脂肪组织功能的预防剂，是预防或改善肥胖及其合并症的合适工具。我们旨在评估具有不同脂肪和免疫调节能力的乳酸菌（LAB）对高脂饮食诱导的小鼠的代谢和免疫参数以及肠道微生物群组成的影响。将Balb/c断奶雄性小鼠喂食标准（SD）或高脂饮食（HFD），并补充或不补充CRL1446（CRL1446）、CRL1434（CRL1434）或CRL431（CRL431），持续45天。评估了SD和HFD小鼠的生化和免疫参数、白色脂肪组织组织学、肠道微生物群组成以及细胞功能（脂肪细胞和巨噬细胞）。与CRL431不同，给予CRL1446和CRL1434会导致体重、脂肪组织重量和脂肪细胞大小发生显著变化。此外，这些菌株导致血浆葡萄糖、胆固醇、甘油三酯、瘦素、TNF-α、IL-6水平降低，IL-10水平升高。在功能测定中，CRL1446和CRL1434肥胖脂肪细胞在LPS刺激后，与肥胖对照组相比，瘦素分泌减少，而CRL431则未观察到变化。在用CRL1446和CRL1434喂养的肥胖小鼠的巨噬细胞中，LPS刺激后，与肥胖对照组相比，MCP-1、TNF-α、IL-6水平较低。相比之下，CRL431未诱导细胞因子值的改变。关于肠道微生物群，所有菌株的施用均导致厚壁菌门/拟杆菌门指数和多样性降低。此外，就属结果而言，所有菌株均增加，主要是属和XIVa属。在研究期间，CRL1446诱导属的增加更高。所测试的益生菌菌株对高脂饮食诱导的肥胖小鼠的肠道微生物群和代谢/免疫参数有不同的调节作用。这些结果表明，CRL1446和CRL1434菌株可作为肥胖和超重营养治疗的辅助益生菌菌株。同时，CRL431菌株在需要调节免疫系统的疾病中可能更有益。
