Byzova Nadezhda A, Safenkova Irina V, Gorbatov Alexey A, Biketov Sergey F, Dzantiev Boris B, Zherdev Anatoly V
A.N. Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia.
State Research Center for Applied Microbiology & Biotechnology, 142279 Obolensk, Russia.
Microorganisms. 2024 Dec 11;12(12):2555. doi: 10.3390/microorganisms12122555.
To ensure the safety of foodstuffs, widespread non-laboratory monitoring for pathogenic contaminants is in demand. A suitable technique for this purpose is lateral flow immunoassay (LFIA) which combines simplicity, rapidity, and productivity with specific immune detection. This study considered three developed formats of LFIA for Typhimurium, a priority pathogenic contaminant of milk. Common sandwich LFIA with all immunoreagents pre-applied to the test strip (format A) was compared with incubation of the sample and (gold nanoparticle-antibody) conjugate, preceding the lateral flow processes (format B), and sequential passages of the sample and the conjugate along the test strip (format C). Under the chosen conditions, the detection limits and the assay times were 3 × 10, 1 × 10, and 3 × 10 cells/mL, 10, 15, and 20 min for formats A, B, and C, respectively. The selected format A of LFIA was successfully applied to test milk samples. The sample's dilution to a fat content of 1.0% causes pathogen detection, with 70-110% revealing and 1.5-8.5% accuracy. The obtained results demonstrate that the developed LFIA allows the detection of lower concentrations of cells and, in this way, accelerates decision-making in food safety control.
为确保食品的安全性，需要对致病性污染物进行广泛的非实验室监测。为此，一种合适的技术是侧向流动免疫分析（LFIA），它将简单性、快速性和高效性与特异性免疫检测相结合。本研究考虑了针对鼠伤寒沙门氏菌（牛奶中的一种优先致病性污染物）的三种已开发的LFIA形式。将所有免疫试剂预先应用于测试条的常见夹心LFIA（形式A）与在侧向流动过程之前对样品和（金纳米颗粒 - 抗体）缀合物进行孵育（形式B）以及样品和缀合物沿测试条顺序通过（形式C）进行了比较。在选定的条件下，形式A、B和C的检测限分别为3×10、1×10和3×10个细胞/mL，检测时间分别为10、15和20分钟。所选择的LFIA形式A成功应用于测试牛奶样品。将样品稀释至脂肪含量为1.0%时可检测到病原体，检出率为70 - 110%，准确率为1.5 - 8.5%。所得结果表明，所开发的LFIA能够检测更低浓度的细胞，从而加快食品安全控制中的决策制定。
