Metallurgy and Materials Science Research Institute, Chulalongkorn University, Soi Chula 12, Phayathai Rd., Pathumwan, Bangkok 10330, Thailand.
Department of Industrial Microbiology, Faculty of Science and Technology, Suan Sunandha Rajabhat University, Nok Street, Dusit, Bangkok 10300, Thailand.
Anal Chem. 2023 Sep 19;95(37):13904-13912. doi: 10.1021/acs.analchem.3c02252. Epub 2023 Aug 28.
Foodborne illnesses caused by the ingestion of contaminated foods or beverages are a serious concern due to the millions of reported cases per year. It is essential to develop sensitive and rapid detection methods of foodborne pathogens to ensure food safety for producers and consumers. Unfortunately, current detection techniques still suffer from time-consuming operations and the need for highly skilled personnel. Here, we introduce a highly sensitive dual colorimetric/electrochemical detection approach for serovar typhimurium ( typhimurium) based on a laser-induced graphene-integrated lateral flow immunoassay (LIG-LFIA) strip. The LIG electrode was fabricated by laser engraving on a polyimide tape containing a pseudo silver/silver chloride reference electrode from silver sintering and chlorination. Using double-sided tape inserted into the strip, automatic sequential reagent delivery was enabled for the dual-mode signal readout by single-sample loading. A gold-deposited gold nanoparticle strategy was first employed to simultaneously obtain a colorimetric signal for early screening and a signal turn-on electrochemical response for high-sensitivity and -quantitative analysis. A superior performance of the strip was established, characterized by a short analysis time (12 min assay +15 min sample preparation), a broad working concentration range (1 cfu/10 mL to 10 cfu/mL), and the lowest limit of detection (1 ± 0.5 cfu/10 mL; mean ± standard deviation, = 3) among reported multimode typhimurium detection schemes. The strip was successfully applied in the analysis of various food products without any bacterial enrichment or amplification required, and the results were comparable to those of the standard culture method.
食源性疾病是由摄入受污染的食物或饮料引起的，每年报告的病例数以百万计，因此这是一个严重的问题。开发敏感和快速的食源性病原体检测方法对于生产者和消费者的食品安全至关重要。不幸的是，目前的检测技术仍然存在操作耗时和需要高技能人员的问题。在这里，我们介绍了一种基于激光诱导石墨烯集成侧向流动免疫分析（LIG-LFIA）条的灵敏双比色/电化学检测方法，用于检测鼠伤寒沙门氏菌（鼠伤寒沙门氏菌）。LIG 电极是通过在聚酰亚胺带上进行激光雕刻制造的，聚酰亚胺带中含有从银烧结和氯化过程中得到的伪银/氯化银参比电极。通过将双面胶带插入条带中，自动顺序试剂输送可实现单一样品加载的双模式信号读出。首先采用金沉积金纳米颗粒策略，同时获得用于早期筛选的比色信号和用于高灵敏度和定量分析的信号开启电化学响应。该条带具有优越的性能，分析时间短（12 分钟测定+15 分钟样品制备），工作浓度范围宽（1 cfu/10 mL 至 10 cfu/mL），检测下限最低（1 ± 0.5 cfu/10 mL；平均值±标准偏差， = 3），与报道的多模式鼠伤寒沙门氏菌检测方案相比。该条带无需进行任何细菌富集或扩增即可成功应用于各种食品产品的分析，结果与标准培养方法相当。
