Ko Hee Jun, Lee Sung Bin, Kim Kee Hoon, Jeon So Young, Jung Ji Yun, Choi Yun Mi, Park Se Chang
Laboratory of Aquatic Biomedicine, Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 08826, Republic of Korea.
Lotteworld Aquarium, Seoul 05551, Republic of Korea.
Microorganisms. 2024 Dec 12;12(12):2558. doi: 10.3390/microorganisms12122558.
Unlike other microbothriid monogenean infections in elasmobranchs, limited information is available on the biology and treatment of . As parasitic infection with was found in 21 juvenile blacktip reef sharks () at the Lotteworld aquarium in Seoul, South Korea, we aimed to investigate the anatomical features and treatment protocols for in this study. The parasites were sampled and fixed in 10% neutral-buffered formalin, and examined using light and scanning electron microscopy. The treatments included short-term praziquantel baths, freshwater immersion, and long-term trichlorfon baths. Manual removal of parasites was also attempted. Examination and manual removal revealed that, similar to other microbothriids, attaches to the placoid scale using opisthaptoral secretion in the posterior region. The effectiveness of the treatments varied, with trichlorfon proving the most effective and safe option for complete parasite eradication. Praziquantel facilitated parasite removal by weakening their attachment, suggesting the potential for higher doses and prolonged exposure times for enhanced antiparasitic effects. To our knowledge, this is the first study providing the microscopic details of infection and its treatment outcomes in captive blacktip reef sharks, thereby providing valuable insights for future research and management.
与板鳃亚纲动物中其他微口吸虫单殖吸虫感染不同，关于[寄生虫名称未给出]的生物学和治疗的信息有限。由于在韩国首尔乐天世界水族馆的21条幼年黑鳍礁鲨（[鲨鱼学名未给出]）中发现了[寄生虫名称未给出]的寄生感染，我们旨在本研究中调查[寄生虫名称未给出]的解剖特征和治疗方案。将寄生虫取样并固定在10%中性缓冲福尔马林中，然后使用光学显微镜和扫描电子显微镜进行检查。治疗方法包括短期吡喹酮药浴、淡水浸泡和长期敌百虫药浴。还尝试手动清除寄生虫。检查和手动清除发现，与其他微口吸虫一样，[寄生虫名称未给出]利用后部区域的后吸器分泌物附着在盾鳞上。治疗效果各不相同，敌百虫被证明是完全根除寄生虫最有效和最安全的选择。吡喹酮通过削弱寄生虫的附着促进了寄生虫的清除，这表明增加剂量和延长暴露时间可能会增强抗寄生虫效果。据我们所知，这是第一项提供圈养黑鳍礁鲨中[寄生虫名称未给出]感染的微观细节及其治疗结果的研究，从而为未来的研究和管理提供了有价值的见解。
