Rusak Agnieszka, Mrozowska Monika, Kozłowska Weronika, Wiatrak Benita, Dzięgiel Piotr, Wybraniec Sławomir, Carev Ivana, Jama-Kmiecik Agnieszka, Matkowski Adam, Zielińska Sylwia
Division of Histology and Embryology, Department of Human Morphology and Embryology, Faculty of Medicine, Wroclaw Medical University, Chałubińskiego 6a, 50-368 Wroclaw, Poland.
Division of Pharmaceutical Biotechnology, Department of Pharmaceutical Biology and Biotechnology, Faculty of Pharmacy, Wroclaw Medical University, Borowska 211, 50-556 Wroclaw, Poland.
Nutrients. 2024 Dec 20;16(24):4393. doi: 10.3390/nu16244393.
L. (Malabar spinach, Basellaceae), widely consumed as a leafy vegetable, produces dark-colored fruits rich in betacyanins, including rare 6-glycosylated derivatives called gomphrenins. Comprehensive studies on the anti-inflammatory potential of its gomphrenin fraction (A) and crude extract (B) employed various analytical and biological methods. Cytotoxicity and anti-inflammatory effects were tested on human and animal cell models using SRB, DCF-DA, Griess, MDA, and ELISA assays. Both the crude extract and enriched gomphrenin fraction exhibited significant anti-inflammatory and antioxidant effects in vitro. They inhibited pro-inflammatory cytokines IL-1β and IL-6, reduced oxidative stress markers (ROS, NO), and decreased lipid peroxidation. The enriched gomphrenin fraction (A) showed stronger antioxidant and anti-inflammatory effects, particularly in reducing ROS and NO levels, though not always concentration-dependent. Both A and B inhibited NF-κB and COX activity. These findings highlight the therapeutic potential of f. fruit extract and betalain fraction for inflammation-related conditions, encouraging further exploration of their mechanisms and application.
落葵（落葵科）作为一种叶菜类蔬菜被广泛食用，其果实颜色深，富含甜菜色素，包括名为紫茉莉苷的罕见6-糖基化衍生物。对其紫茉莉苷组分（A）和粗提物（B）的抗炎潜力进行的综合研究采用了各种分析和生物学方法。使用SRB、DCF-DA、Griess、MDA和ELISA测定法在人和动物细胞模型上测试了细胞毒性和抗炎作用。粗提物和富集的紫茉莉苷组分在体外均表现出显著的抗炎和抗氧化作用。它们抑制促炎细胞因子IL-1β和IL-6，降低氧化应激标志物（ROS、NO），并减少脂质过氧化。富集的紫茉莉苷组分（A）表现出更强的抗氧化和抗炎作用，尤其是在降低ROS和NO水平方面，不过并不总是呈浓度依赖性。A和B均抑制NF-κB和COX活性。这些发现突出了落葵果实提取物和甜菜色素组分对炎症相关病症的治疗潜力，鼓励进一步探索其作用机制和应用。
