Yi Huan, Zhao Yarui, Wu Xinting, Zhang Mengting, Pan Minwei, Ding Siyuan, Huang Jiaqi, Gu Qing, Zha Xiaojun
College of Life Sciences, Zhejiang Normal University, 688 Yingbin Road, Jinhua 321004, China.
Plants (Basel). 2024 Dec 21;13(24):3569. doi: 10.3390/plants13243569.
Low temperature affects rice growth and yield. Receptor-like protein kinases play an important role in plant growth and development. In order to reveal the role of a leucine-rich receptor like kinase LRK2 in low temperature stress and growth and development of rice. In this study, we used the obtained overexpressing plants for experiments and the results show that the cold tolerance and yield of overexpressing plants were higher than that of wild type. has high homology with the phytosulfokine receptors (PSKRs) gene of different species, and gene is responsive to phytosulfokine (PSK). In addition, we observed that the proline content of overexpressing plants was significantly higher than that of the wild-type at low temperature, while the malondialdehyde content was significantly lower than that of the wild-type. Yeast two-hybrid screening and bimolecular fluorescence complementary analysis showed that LRK2 interacts with rice growth hormone response factor 24 (OsARF24) in vitro. These results suggest that gene may be involved in the regulation of cold stress response and yield in rice. These findings will help us understand PSKR signaling in other grasses and support improvements in rice genetics.
低温影响水稻生长和产量。类受体蛋白激酶在植物生长发育中起重要作用。为揭示富含亮氨酸的类受体激酶LRK2在水稻低温胁迫及生长发育中的作用。在本研究中，我们利用获得的过表达植株进行实验，结果表明过表达植株的耐寒性和产量高于野生型。与不同物种的植物硫肽激素受体（PSKRs）基因具有高度同源性，且该基因对植物硫肽激素（PSK）有响应。此外，我们观察到在低温条件下，过表达植株的脯氨酸含量显著高于野生型，而丙二醛含量显著低于野生型。酵母双杂交筛选和双分子荧光互补分析表明，LRK2在体外与水稻生长激素响应因子24（OsARF24）相互作用。这些结果表明该基因可能参与水稻冷胁迫响应和产量的调控。这些发现将有助于我们了解其他禾本科植物中的PSKR信号传导，并支持水稻遗传学的改良。
