Department of Biological Sciences, College of Science, King Faisal University, P.O. Box 380, Al-Ahsa 31982, Saudi Arabia.
Department of Genetics, Faculty of Agriculture, Ain Shams University, 68 Hadayek Shoubra, Cairo 11241, Egypt.
Genes (Basel). 2024 Oct 9;15(10):1306. doi: 10.3390/genes15101306.
The phytosulfokine receptor () gene family plays a crucial role in regulating plant growth, development, and stress response. Here, the gene family was characterized in L. The study aimed to bridge knowledge gaps and clarify the functional roles of to create a solid foundation for examining the structure, functions, and regulatory aspects. The investigation involved genome-wide identification of through collection and chromosomal assignment, followed by phylogenetic analysis and gene expression profiling. Additionally, interactions with their interactors were stimulated and analyzed to elucidate their function. The wide-genome inspection of all led to 25 genes with various homeologs, resulting in 57 members distributed among the A, B, and D subgenomes. Investigating the expression of 61 PSKR cDNAs in RNA-seq datasets generated from different growth stages at 14, 21, and 60 days old and diverse tissues such as leaves, shoots, and roots provided further insight into their functional purposes. The expression profile of the resulted in three key clusters. Gene cluster 1 (GC 1) is partially associated with root growth, suggesting that specific s control root development. The GC 2 cluster targeted genes that show high levels of expression in all tested leaf growth stages and the early developmental stage of the shoots and roots. Furthermore, the GC 3 cluster was composed of genes that are constantly expressed, highlighting their crucial role in regulating various processes during the entire life cycle of wheat. Molecular docking simulations showed that phytosulfokine type α (PSK-α) interacted with all PSKRs and had a strong binding affinity with certain PSKR proteins, encompassing PSKR1A, PSKR3B, and PSKR13A, that support their involvement in PSK signaling pathways. The crucial arbitration of the affinity may depend on interactions between wheat PSK-α and PSKRs, especially in the LRR domain region. These discoveries deepened our knowledge of the role of the gene family in wheat growth and development, opening up possibilities for further studies to enhance wheat durability and yield via focused innovation approaches.
植物磺基肽受体（PSKR）基因家族在调节植物生长、发育和应激反应方面发挥着关键作用。在这里，对 L. 进行了特征描述。该研究旨在填补知识空白，阐明 的功能作用，为研究其结构、功能和调控方面奠定坚实基础。该研究通过收集和染色体分配进行了全基因组范围内的 基因家族鉴定，随后进行了系统发育分析和基因表达谱分析。此外，还刺激并分析了与它们的相互作用物的相互作用，以阐明它们的功能。对所有 的广泛基因组检查导致了 25 个具有不同同源物的基因，最终产生了 57 个成员，分布在 A、B 和 D 亚基因组中。研究了 61 个 PSKR cDNA 在来自不同生长阶段（14、21 和 60 天）和不同组织（如叶、茎和根）的 RNA-seq 数据集的表达，进一步深入了解了它们的功能目的。 的表达谱导致了三个关键聚类。基因聚类 1（GC 1）部分与根生长有关，表明特定的 s 控制根发育。GC 2 聚类靶向在所有测试的叶生长阶段以及茎和根的早期发育阶段表现出高水平表达的基因。此外，GC 3 聚类由持续表达的基因组成，突出了它们在小麦整个生命周期中调节各种过程的关键作用。分子对接模拟表明，植物磺基肽型α（PSK-α）与所有 PSKR 相互作用，并且与某些 PSKR 蛋白具有很强的结合亲和力，包括 PSKR1A、PSKR3B 和 PSKR13A，支持它们参与 PSK 信号通路。亲和力的关键仲裁可能取决于小麦 PSK-α与 PSKR 之间的相互作用，尤其是在 LRR 结构域区域。这些发现加深了我们对 PSKR 基因家族在小麦生长和发育中的作用的认识，为通过有针对性的创新方法提高小麦的耐久性和产量的进一步研究开辟了可能性。
